Anti-ETF1 Antibody (CAB5920)
- SKU:
- CAB5920
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-ETF1 Antibody |
| 抗体コード: | CAB5920 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 278-437 of human ETF1 (NP_004721.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Raji, U-87MG, HT-1080, SKOV3, Mouse liver, Mouse heart, Mouse kidney, Rat heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 278-437 of human ETF1 (NP_004721.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | VKFI QEKK LIGR YFDE ISQD TGKY CFGV EDTL KALE MGAV EILI VYEN LDIM RYVL HCQG TEEE KILY LTPE QEKD KSHF TDKE TGQE HELI ESMP LLEW FANN YKKF GATL EIVT DKSQ EGSQ FVKG FGGI GGIL RYRV DFQG MEYQ GGDD EFFD LDDY |
| 遺伝子ID: | 2107 |
| Uniprot: | P62495 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 45kDa/49kDa |
| 観察された分子量: | 55kDa |
| 同義語: | ETF1, D5S1995, ERF, ERF1, RF1, SUP45L1, TB3-1 |
| バックグラウンド: | This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X. |
| UniProt Protein Function: | ETF1: Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Belongs to the eukaryotic release factor 1 family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 5q31.1 Cellular Component: cytoplasm; nucleus; cytosol Molecular Function:protein binding; RNA binding; translation termination factor activity; translation release factor activity; ribosome binding; translation release factor activity, codon specific Biological Process: cellular protein metabolic process; protein amino acid methylation; translation; mRNA catabolic process, nonsense-mediated decay; gene expression; translational termination; regulation of translational termination |
| NCBI Summary: | This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X. [provided by RefSeq, Aug 2013] |
| UniProt Code: | P62495 |
| NCBI GenInfo Identifier: | 50402099 |
| NCBI Gene ID: | 2107 |
| NCBI Accession: | P62495.3 |
| UniProt Secondary Accession: | P62495,P46055, Q5M7Z7, Q96CG1, B2R6B4, D3DQC1, |
| UniProt Related Accession: | P62495 |
| Molecular Weight: | 437 |
| NCBI Full Name: | Eukaryotic peptide chain release factor subunit 1 |
| NCBI Synonym Full Names: | eukaryotic translation termination factor 1 |
| NCBI Official Symbol: | ETF1 |
| NCBI Official Synonym Symbols: | ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1 |
| NCBI Protein Information: | eukaryotic peptide chain release factor subunit 1; protein Cl1; polypeptide chain release factor 1; sup45 (yeast omnipotent suppressor 45) homolog-like 1 |
| UniProt Protein Name: | Eukaryotic peptide chain release factor subunit 1 |
| UniProt Synonym Protein Names: | Protein Cl1; TB3-1 |
| Protein Family: | Probable electron transfer flavoprotein |
| UniProt Gene Name: | ETF1 |
| UniProt Entry Name: | ERF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ETF1 antibody (CAB5920) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunofluorescence analysis of HeLa cells using ETF1 Rabbit pAb (CAB5920) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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