Anti-ERAP2 Antibody (CAB9024)

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SKU:
CAB9024
Product type:
Antibody
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
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Description

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抗体名:Anti-ERAP2 Antibody
抗体コード:CAB9024
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB
反応性:Human
宿主種:Rabbit
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ERAP2 (NP_071745.1).
申し込み:WB
推奨希釈:WB 1:500 - 1:2000
反応性:Human
ポジティブサンプル:A375, HeLa, 293T
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ERAP2 (NP_071745.1).
精製方法:Affinity purification
ストレージバッファ:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:MFHS SAMV NSHR KPMF NIHR GFYC LTAI LPQI CICS QFSV PSSY HFTE DPGA FPVA TNGE RFPW QELR LPSV VIPL HYDL FVHP NLTS LDFV ASEK IEVL VSNA TQFI ILHS KDLE ITNA TLQS EEDS RYMK PGKE LKVL SYPA HEQI ALLV PEKL TPHL KYYV AMDF QA
遺伝子ID:64167
Uniprot:Q6P179
セルラーロケーション:Endoplasmic reticulum membrane, Single-pass type II membrane protein
計算された分子量:40kDa/60kDa/105kDa/110kDa
観察された分子量:110kDa
同義語:ERAP2, L-RAP, LRAP
バックグラウンド:
UniProt Protein Function:ERAP2: Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I- binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Preferentially hydrolyzes the basic residues Arg and Lys. Belongs to the peptidase M1 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Endoplasmic reticulum; Membrane protein, integral; Protease; EC 3.4.11.-

Chromosomal Location of Human Ortholog: 5q15

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum lumen; integral to membrane

Molecular Function:metallopeptidase activity; zinc ion binding; aminopeptidase activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; regulation of blood pressure; proteolysis; antigen processing and presentation of endogenous peptide antigen via MHC class I

NCBI Summary:Aminopeptidases hydrolyze N-terminal amino acids of proteins or peptide substrates. Major histocompatibility complex (MHC) class I molecules rely on aminopeptidases such as ERAP1 (MIM 606832) and LRAP to trim precursors to antigenic peptides in the endoplasmic reticulum (ER) following cleavage in the cytoplasm by tripeptidyl peptidase II (TPP2; MIM 190470) (Tanioka et al., 2003 [PubMed 12799365]).[supplied by OMIM, Mar 2008]
UniProt Code:Q6P179
NCBI GenInfo Identifier:194306629
NCBI Gene ID:64167
NCBI Accession:NP_001123612.1
UniProt Secondary Accession:Q6P179,Q7Z5K1, Q8TD32, Q8WVJ4, Q9HBX2,
UniProt Related Accession:Q6P179
Molecular Weight:Predicted: 30.0kDaAccurate: 34kDa as determined by SDS-PAGE reducing conditions.
NCBI Full Name:endoplasmic reticulum aminopeptidase 2
NCBI Synonym Full Names:endoplasmic reticulum aminopeptidase 2
NCBI Official Symbol:ERAP2  
NCBI Official Synonym Symbols:LRAP; L-RAP  
NCBI Protein Information:endoplasmic reticulum aminopeptidase 2
UniProt Protein Name:Endoplasmic reticulum aminopeptidase 2
UniProt Synonym Protein Names:Leukocyte-derived arginine aminopeptidase; L-RAP
Protein Family:Endoplasmic reticulum aminopeptidase
UniProt Gene Name:ERAP2  
UniProt Entry Name:ERAP2_HUMAN
Anti-ERAP2 Polyclonal Antibody (CAB9024)Western blot analysis of extracts of various cell lines, using ERAP2 antibody (CAB9024) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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