Anti-ERAL1 Antibody (CAB10382)
- SKU:
- CAB10382
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ERAL1 Antibody |
| 抗体コード: | CAB10382 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 198-437 of human ERAL1 (NP_005693.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, 293T |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 198-437 of human ERAL1 (NP_005693.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | DLVV VLVD VSDK WTRN QLSP QLLR CLTK YSQI PSVL VMNK VDCL KQKS VLLE LTAA LTEG VVNG KKLK MRQA FHSH PGTH CPSP AVKD PNTQ SVGN PQRI GWPH FKEI FMLS ALSQ EDVK TLKQ YLLT QAQP GPWE YHSA VLTS QTPE EICA NIIR EKLL EHLP QEVP YNVQ QKTA VWEE GPGG ELVI QQKL LVPK ESYV KLLI GPKG HVIS QIAQ EAGH DLMD IFLC DVDI RLSV KLLK |
| 遺伝子ID: | 26284 |
| Uniprot: | O75616 |
| セルラーロケーション: | Mitochondrion inner membrane, Mitochondrion matrix, Peripheral membrane protein |
| 計算された分子量: | 30kDa/48kDa |
| 観察された分子量: | 48KDa |
| 同義語: | ERAL1, CEGA, ERA, ERA-W, ERAL1A, H-ERA, HERA-A, HERA-B, GTPase Era |
| バックグラウンド: | The protein encoded by this gene is a GTPase that localizes to the mitochondrion. The encoded protein binds to the 3' terminal stem loop of 12S mitochondrial rRNA and is required for proper assembly of the 28S small mitochondrial ribosomal subunit. Deletion of this gene has been shown to cause mitochondrial dysfunction, growth retardation, and apoptosis. Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ERAL1: Probable GTPase that plays a role in the mitochondrial ribosomal small subunit assembly. Specifically binds the 12S mitochondrial rRNA (12S mt-rRNA) to a 33 nucleotide section delineating the 3' terminal stem-loop region. May act as a chaperone that protects the 12S mt-rRNA on the 28S mitoribosomal subunit during ribosomal small subunit assembly. Belongs to the Era/MnmE GTP-binding protein family. Era subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial Chromosomal Location of Human Ortholog: 17q11.2 Cellular Component: actin cytoskeleton; cytoplasm; intermediate filament cytoskeleton; mitochondrial matrix; mitochondrion Molecular Function:protein binding; ribosomal small subunit binding; rRNA binding Biological Process: ribosomal small subunit assembly and maintenance |
| NCBI Summary: | The protein encoded by this gene is a GTPase that localizes to the mitochondrion. The encoded protein binds to the 3' terminal stem loop of 12S mitochondrial rRNA and is required for proper assembly of the 28S small mitochondrial ribosomal subunit. Deletion of this gene has been shown to cause mitochondrial dysfunction, growth retardation, and apoptosis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015] |
| UniProt Code: | O75616 |
| NCBI GenInfo Identifier: | 57015286 |
| NCBI Gene ID: | 26284 |
| NCBI Accession: | O75616.2 |
| UniProt Secondary Accession: | O75616,O75617, Q8WUY4, Q96LE2, Q96TC0, B3KN21, C9JEC6 |
| UniProt Related Accession: | O75616 |
| Molecular Weight: | 30,040 Da |
| NCBI Full Name: | GTPase Era, mitochondrial |
| NCBI Synonym Full Names: | Era like 12S mitochondrial rRNA chaperone 1 |
| NCBI Official Symbol: | ERAL1 |
| NCBI Official Synonym Symbols: | ERA; CEGA; ERA-W; H-ERA; ERAL1A; HERA-A; HERA-B |
| NCBI Protein Information: | GTPase Era, mitochondrial |
| UniProt Protein Name: | GTPase Era, mitochondrial |
| UniProt Synonym Protein Names: | Conserved ERA-like GTPase; CEGA; ERA-W; ERA-like protein 1 |
| UniProt Gene Name: | ERAL1 |
| UniProt Entry Name: | ERAL1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ERAL1 Rabbit pAb (CAB10382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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