Anti-EPOR Antibody (CAB2917)
- SKU:
- CAB2917
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-EPOR Antibody |
| 抗体コード: | CAB2917 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 274-508 of human EPOR (NP_000112.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | K-562, Mouse kidney, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 274-508 of human EPOR (NP_000112.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | HRRA LKQK IWPG IPSP ESEF EGLF TTHK GNFQ LWLY QNDG CLWW SPCT PFTE DPPA SLEV LSER CWGT MQAV EPGT DDEG PLLE PVGS EHAQ DTYL VLDK WLLP RNPP SEDL PGPG GSVD IVAM DEGS EASS CSSA LASK PSPE GASA ASFE YTIL DPSS QLLR PWTL CPEL PPTP PHLK YLYL VVSD SGIS TDYS SGDS QGAQ GGLS DGPY SNPY ENSL IPAA EPLP PSYV ACS |
| 遺伝子ID: | 2057 |
| Uniprot: | P19235 |
| セルラーロケーション: | Cell membrane, Secreted, Single-pass type I membrane protein |
| 計算された分子量: | 26kDa/35kDa/55kDa |
| 観察された分子量: | 55kDa |
| 同義語: | EPOR, EPO-R |
| バックグラウンド: | This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants. |
| UniProt Protein Function: | EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling. |
| UniProt Protein Details: | Protein type:Receptor, cytokine; Membrane protein, integral Chromosomal Location of Human Ortholog: 19p13.3-p13.2 Cellular Component: integral to plasma membrane Molecular Function:erythropoietin receptor activity; identical protein binding; protein binding Disease: Erythrocytosis, Familial, 1 |
| NCBI Summary: | This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010] |
| UniProt Code: | P19235 |
| NCBI GenInfo Identifier: | 119524 |
| NCBI Gene ID: | 2057 |
| NCBI Accession: | P19235.1 |
| UniProt Secondary Accession: | P19235,Q15443, Q2M205, B2RCG4, |
| UniProt Related Accession: | P19235 |
| Molecular Weight: | 35,809 Da |
| NCBI Full Name: | Erythropoietin receptor |
| NCBI Synonym Full Names: | erythropoietin receptor |
| NCBI Official Symbol: | EPOR |
| NCBI Official Synonym Symbols: | EPO-R |
| NCBI Protein Information: | erythropoietin receptor |
| UniProt Protein Name: | Erythropoietin receptor |
| Protein Family: | Erythropoietin receptor |
| UniProt Gene Name: | EPOR |
| UniProt Entry Name: | EPOR_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EPOR antibody (CAB2917) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
 | Immunohistochemistry of paraffin-embedded human prostate using EPOR antibody (CAB2917) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human trachea using EPOR antibody (CAB2917) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of Mouse fetal using EPOR Rabbit pAb (CAB2917) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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