Anti-EML4 抗体 (CAB16117)

抗体名:	Anti-EML4 Antibody
抗体コード:	CAB16117
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IF
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 360-560 of human EML4 (NP_061936.2).

申し込み:	WB IHC IF
推奨希釈:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
反応性:	Human, Mouse, Rat
ポジティブサンプル:	HepG2

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 360-560 of human EML4 (NP_061936.2).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	GTFE RGVG CLDF SKAD SGVH LCVI DDSN EHML TVWD WQKK AKGA EIKT TNEV VLAV EFHP TDAN TIIT CGKS HIFF WTWS GNSL TRKQ GIFG KYEK PKFV QCLA FLGN GDVL TGDS GGVM LIWS KTTV EPTP GKGP KGVY QISK QIKA HDGS VFTL CQMR NGML LTGG GKDR KIIL WDHD LNPE REIE VPDQ YGTI RAVA E
遺伝子ID:	27436
Uniprot:	Q9HC35
セルラーロケーション:	Cytoplasm, cytoskeleton
計算された分子量:	102kDa/108kDa
観察された分子量:	120kDa

同義語:

EML4, C2orf2, ELP120, EMAP-4, EMAPL4, ROPP120

バックグラウンド:

This gene is a member of the echinoderm microtubule associated protein-like family. The encoded WD-repeat protein may be involved in microtubule formation. Abnormal fusion of parts of this gene with portions of the anaplastic lymphoma receptor tyrosine kinase gene, which generates EML4-ALK fusion transcripts, is one of the primary mutations associated with non-small cell lung cancer. Alternative splicing of this gene results in two transcript variants.

UniProt Protein Function:	EML4: a microtubule-associated protein. A WD-repeat protein associated with the mitotic spindle apparatus. May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic. Strongly overexpressed during mitosis.
UniProt Protein Details:	Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 2p21 Cellular Component: membrane; microtubule cytoskeleton Molecular Function:microtubule binding Biological Process: microtubule cytoskeleton organization and biogenesis
NCBI Summary:	This gene is a member of the echinoderm microtubule associated protein-like family. The encoded WD-repeat protein may be involved in microtubule formation. Abnormal fusion of parts of this gene with portions of the anaplastic lymphoma receptor tyrosine kinase gene, which generates EML4-ALK fusion transcripts, is one of the primary mutations associated with non-small cell lung cancer. Alternative splicing of this gene results in two transcript variants. [provided by RefSeq, Jan 2015]
UniProt Code:	Q9HC35
NCBI GenInfo Identifier:	14250486
NCBI Gene ID:	27436
NCBI Accession:	BC008685
UniProt Secondary Accession:	Q9HC35,Q3SWW0, Q53R29, Q53TW8, Q6PJ45, Q9NV40, A6H8Y6 B2RBK3, B2RTW7, B5MCW9,
UniProt Related Accession:	Q9HC35
Molecular Weight:	102,444 Da
NCBI Full Name:	Homo sapiens echinoderm microtubule associated protein like 4, mRNA
NCBI Synonym Full Names:	echinoderm microtubule associated protein like 4
NCBI Official Symbol:	EML4
NCBI Official Synonym Symbols:	C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120
NCBI Protein Information:	echinoderm microtubule-associated protein-like 4
UniProt Protein Name:	Echinoderm microtubule-associated protein-like 4
UniProt Synonym Protein Names:	Restrictedly overexpressed proliferation-associated protein; Ropp 120
Protein Family:	Echinoderm microtubule-associated protein
UniProt Gene Name:	EML4
UniProt Entry Name:	EMAL4_HUMAN

	Western blot analysis of extracts of HepG2 cells, using EML4 Rabbit pAb (CAB16117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded Mouse kidney using EML4 Rabbit pAb (CAB16117) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat kidney using EML4 Rabbit pAb (CAB16117) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human liver cancer using EML4 Rabbit pAb (CAB16117) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using EML4 antibody (CAB16117) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)