Anti-EIF4H Antibody (CAB9953)
- SKU:
- CAB9953
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-EIF4H Antibody |
| 抗体コード: | CAB9953 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human EIF4H (NP_114381.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | SH-SY5Y, Jurkat, HeLa, SW480, Mouse pancreas |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human EIF4H (NP_114381.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MADF DTYD DRAY SSFG GGRG SRGS AGGH GSRS QKEL PTEP PYTA YVGN LPFN TVQG DIDA IFKD LSIR SVRL VRDK DTDK FKGF CYVE FDEV DSLK EALT YDGA LLGD RSLR VDIA EGRK QDKG GFGF RKGG PDDR GFRD DFLG GRGG SRPG DRRT GPPM GSRF RDGP PLRG SNMD FREP TEEE RAQR PRLQ LKPR TVAT PLNQ VANP NSAI FGGA RPRE EVVQ KEQE |
| 遺伝子ID: | 7458 |
| Uniprot: | Q15056 |
| セルラーロケーション: | Cytoplasm, perinuclear region |
| 計算された分子量: | 25kDa/27kDa |
| 観察された分子量: | 27kDa |
| 同義語: | EIF4H, WBSCR1, WSCR1, eIF-4H |
| バックグラウンド: | This gene encodes one of the translation initiation factors, which functions to stimulate the initiation of protein synthesis at the level of mRNA utilization. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene generates 2 transcript variants. |
| UniProt Protein Function: | EIF4H: eukaryotic translation initiation factor 4H stimulates protein translation. May stimulate the activities of EIF4A, EIF4B and EIF4F during the steps involving mRNA binding and utilization in the initiation of protein biosynthesis. Binds mRNA. Two alternatively spliced isoforms have been described. The short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney. |
| UniProt Protein Details: | Protein type:Translation initiation; Translation; RNA-binding Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: cytosol; eukaryotic translation initiation factor 4F complex; membrane; polysome Molecular Function:helicase activity; protein binding; ribosomal small subunit binding; RNA binding; RNA strand annealing activity; RNA strand-exchange activity; translation factor activity, nucleic acid binding Biological Process: formation of translation preinitiation complex; regulation of translational initiation; translational initiation Disease: Williams-beuren Syndrome |
| NCBI Summary: | This gene encodes one of the translation initiation factors, which functions to stimulate the initiation of protein synthesis at the level of mRNA utilization. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15056 |
| NCBI GenInfo Identifier: | 18276665 |
| NCBI Gene ID: | 7458 |
| NCBI Accession: | Q15056.5 |
| UniProt Secondary Accession: | Q15056,A8K3R1, D3DXF6, D3DXF8, |
| UniProt Related Accession: | Q15056 |
| Molecular Weight: | 25,200 Da |
| NCBI Full Name: | Eukaryotic translation initiation factor 4H |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 4H |
| NCBI Official Symbol: | EIF4H |
| NCBI Official Synonym Symbols: | WSCR1; WBSCR1; eIF-4H |
| NCBI Protein Information: | eukaryotic translation initiation factor 4H |
| UniProt Protein Name: | Eukaryotic translation initiation factor 4H |
| UniProt Synonym Protein Names: | Williams-Beuren syndrome chromosomal region 1 protein |
| Protein Family: | Eukaryotic translation initiation factor |
| UniProt Gene Name: | EIF4H |
| UniProt Entry Name: | IF4H_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EIF4H antibody (CAB9953) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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