Anti-EIF3D Antibody (CAB5947)
- SKU:
- CAB5947
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-EIF3D Antibody |
| 抗体コード: | CAB5947 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human EIF3D (NP_003744.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | SGC-7901, U-87MG, HeLa, NIH/3T3, Mouse liver, Rat testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human EIF3D (NP_003744.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAKF MTPV IQDN PSGW GPCA VPEQ FRDM PYQP FSKG DRLG KVAD WTGA TYQD KRYT NKYS SQFG GGSQ YAYF HEED ESSF QLVD TART QKTA YQRN RMRF AQRN LRRD KDRR NMLQ FNLQ ILPK SAKQ KERE RIRL QKKF QKQF GVRQ KWDQ KSQK PRDS SVEV RSDW EVKE EMDF PQLM KMRY LEVS EPQD IECC GALE YYDK AFDR ITTR SEKP LRSI KRIF HTVT TTDD PVIR KLAK |
| 遺伝子ID: | 8664 |
| Uniprot: | O15371 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 58kDa/62kDa/63kDa |
| 観察された分子量: | 64kDa |
| 同義語: | EIF3D, EIF3S7, eIF3-p66, eIF3-zeta |
| バックグラウンド: | Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. |
| UniProt Protein Function: | eIF3-zeta: eukaryotic translation initiation factor 3 subunit 7. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. Associates with the subunit p170 of eIF-3. eIF-3 is composed of at least 12 different subunits. |
| UniProt Protein Details: | Protein type:Translation; Translation initiation; RNA-binding Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: cytosol; eukaryotic translation initiation factor 3 complex; membrane Molecular Function:protein binding; translation initiation factor activity Biological Process: cellular protein metabolic process; formation of translation initiation complex; formation of translation preinitiation complex; gene expression; regulation of translational initiation; translation; translational initiation |
| NCBI Summary: | Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O15371 |
| NCBI GenInfo Identifier: | 6685526 |
| NCBI Gene ID: | 8664 |
| NCBI Accession: | O15371.1 |
| UniProt Secondary Accession: | O15371,Q3MJD9, Q5M9Q6, A8MWD3, B2R7D4, B4DTF8, B4DVY1 |
| UniProt Related Accession: | O15371 |
| Molecular Weight: | 62,040 Da |
| NCBI Full Name: | Eukaryotic translation initiation factor 3 subunit D |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 3 subunit D |
| NCBI Official Symbol: | EIF3D |
| NCBI Official Synonym Symbols: | EIF3S7; eIF3-p66; eIF3-zeta |
| NCBI Protein Information: | eukaryotic translation initiation factor 3 subunit D |
| UniProt Protein Name: | Eukaryotic translation initiation factor 3 subunit D |
| UniProt Synonym Protein Names: | Eukaryotic translation initiation factor 3 subunit 7 |
| UniProt Gene Name: | EIF3D |
| UniProt Entry Name: | EIF3D_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EIF3D antibody (CAB5947) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of L-929 cells using EIF3D antibody (CAB5947) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of C6 cells using EIF3D antibody (CAB5947) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L-929 cells using EIF3D antibody (CAB5947) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using EIF3D antibody (CAB5947) at dilution of 1:100. Blue: DAPI for nuclear staining. |