Anti-EHD2 Antibody (CAB12945)
- SKU:
- CAB12945
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-EHD2 Antibody |
| 抗体コード: | CAB12945 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 244-543 of human EHD2 (NP_055416.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:1000 - 1:3000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | A-549, U-87MG, HeLa, Mouse lung, Rat lung |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 244-543 of human EHD2 (NP_055416.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | VGTP EVLR VYIG SFWS QPLL VPDN RRLF ELEE QDLF RDIQ GLPR HAAL RKLN DLVK RARL VRVH AYII SYLK KEMP SVFG KENK KKQL ILKL PVIF AKIQ LEHH ISPG DFPD CQKM QELL MAHD FTKF HSLK PKLL EALD EMLT HDIA KLMP LLRQ EELE STEV GVQG GAFE GTHM GPFV ERGP DEAM EDGE EGSD DEAE WVVT KDKS KYDE IFYN LAPA DGKL SGSK AKTW MVGT KLPN SVLG RIWK LSDV DRDG MLDD EEFA LASH LIEA KLEG HGLP ANLP RRLV PPSK RRHK GSAE |
| 遺伝子ID: | 30846 |
| Uniprot: | Q9NZN4 |
| セルラーロケーション: | Cell membrane, Cytoplasm, Cytoplasmic side, Endosome membrane, Membrane, Peripheral membrane protein, caveola, cytosol |
| 計算された分子量: | 46kDa/61kDa |
| 観察された分子量: | 61kDa |
| 同義語: | EHD2, PAST2 |
| バックグラウンド: | This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. |
| UniProt Protein Function: | EHD2: Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: caveola; cytosol; endosome membrane; extrinsic to membrane; nucleus; plasma membrane; recycling endosome membrane Molecular Function:nucleic acid binding; protein binding Biological Process: blood coagulation; endocytic recycling |
| NCBI Summary: | This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NZN4 |
| NCBI GenInfo Identifier: | 57015322 |
| NCBI Gene ID: | 30846 |
| NCBI Accession: | Q9NZN4.2 |
| UniProt Secondary Accession: | Q9NZN4,Q96CB6, B2RDH9, B4DNU6, |
| UniProt Related Accession: | Q9NZN4 |
| Molecular Weight: | 46,059 Da |
| NCBI Full Name: | EH domain-containing protein 2 |
| NCBI Synonym Full Names: | EH domain containing 2 |
| NCBI Official Symbol: | EHD2 |
| NCBI Official Synonym Symbols: | PAST2 |
| NCBI Protein Information: | EH domain-containing protein 2 |
| UniProt Protein Name: | EH domain-containing protein 2 |
| UniProt Synonym Protein Names: | PAST homolog 2Curated |
| Protein Family: | EH domain-containing protein |
| UniProt Gene Name: | EHD2 |
| UniProt Entry Name: | EHD2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EHD2 antibody (CAB12945) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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