Anti-EGF Antibody (CAB13615)
- SKU:
- CAB13615
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-EGF Antibody |
| 抗体コード: | CAB13615 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 850-950 of human EGF (NP_001954.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | 293T, NCI-H460, COS7 |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 850-950 of human EGF (NP_001954.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | GEDA TCQC LKGF AGDG KLCS DIDE CEMG VPVC PPAS SKCI NTEG GYVC RCSE GYQG DGIH CLDI DECQ LGEH SCGE NASC TNTE GGYT CMCA GRLS EPGL I |
| 遺伝子ID: | 1950 |
| Uniprot: | P01133 |
| セルラーロケーション: | Membrane, Single-pass type I membrane protein |
| 計算された分子量: | 129kDa/133kDa |
| 観察された分子量: | 134kDa |
| 同義語: | EGF, HOMG4, URG |
| バックグラウンド: | This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. |
| NCBI Summary: | This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016] |
| UniProt Code: | P01133 |
| NCBI GenInfo Identifier: | 251757262 |
| NCBI Gene ID: | 1950 |
| NCBI Accession: | P01133.2 |
| UniProt Related Accession: | P01133 |
| Molecular Weight: | 6.2 |
| NCBI Full Name: | Pro-epidermal growth factor |
| NCBI Synonym Full Names: | epidermal growth factor |
| NCBI Official Symbol: | EGF |
| NCBI Official Synonym Symbols: | URG; HOMG4 |
| NCBI Protein Information: | pro-epidermal growth factor |
| UniProt Protein Name: | Pro-epidermal growth factor |
| UniProt Synonym Protein Names: | Urogastrone |
| Protein Family: | Epidermal growth factor |
| UniProt Gene Name: | EGF |
| UniProt Entry Name: | EGF_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EGF antibody (CAB13615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of human skin using EGF Rabbit pAb (CAB13615) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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