Anti-EDC4 Antibody (CAB15418)
- SKU:
- CAB15418
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-EDC4 Antibody |
| 抗体コード: | CAB15418 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 800-1100 of human EDC4 (NP_055144.3). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:200 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | Jurkat, Mouse liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 800-1100 of human EDC4 (NP_055144.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | GDGD RHNT PSLL EAAL TQEA STPD SQVW PTAP DITR ETCS TLAE SPRN GLQE KHKS LAFH RPPY HLLQ QRDS QDAS AEQS DHDD EVAS LASA SGGF GTKV PAPR LPAK DWKT KGSP RTSP KLKR KSKK DDGD AAMG SRLT EHQV AEPP EDWP ALIW QQQR ELAE LRHS QEEL LQRL CTQL EGLQ STVT GHVE RALE TRHE QEQR RLER ALAE GQQR GGQL QEQL TQQL SQAL SSAV AGRL ERSI RDEI KKTV PPCV SRSL EPMA GQLS NSVA TKLT AVEG SMKE NISK LLKS KNLT DAIA R |
| 遺伝子ID: | 23644 |
| Uniprot: | Q6P2E9 |
| セルラーロケーション: | Cytoplasm, Nucleus, P-body |
| 計算された分子量: | 109kDa/151kDa |
| 観察された分子量: | 160-180kDa |
| 同義語: | EDC4, GE1, Ge-1, HEDL5, HEDLS, RCD-8, RCD8 |
| バックグラウンド: |
| UniProt Protein Function: | EDC4: a protein required for the 5' a?? 3' pathway of mRNA decapping and degradation-based post-transcriptional gene silencing. The proteins involved in 5' a?? 3' mRNA decay include DCP2, DCP1A, EDC3, EDC4 and DDX6, and are concentrated in cytoplasmic structures called mRNA "processing bodies" (P-bodies). Shortening of the 3'-poly(A) tail and removal of PABP precedes cleavage of the 5'-mRNA cap by a complex containing decapping enzymes 1A and 2 (DCP1A/DCP2). EDC4 enhances the catalytic activity of DCP2 in vitro. Shown to be required for silencing by miRNAs in Drosophila melanogaster. Two alternatively spliced human isoforms have been described. |
| UniProt Protein Details: | Protein type:RNA processing Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: nucleoplasm; membrane; intracellular membrane-bound organelle; cytoplasm; nucleus; cytosol Molecular Function:protein binding Biological Process: gene expression; mRNA catabolic process, deadenylation-dependent decay |
| UniProt Code: | Q6P2E9 |
| NCBI GenInfo Identifier: | 74758241 |
| NCBI Gene ID: | 23644 |
| NCBI Accession: | Q6P2E9.1 |
| UniProt Secondary Accession: | Q6P2E9,Q13025, Q13826, Q6ZR12, Q7Z6H7, A6NGM1, A8K4T4 |
| UniProt Related Accession: | Q6P2E9 |
| Molecular Weight: | 151,661 Da |
| NCBI Full Name: | Enhancer of mRNA-decapping protein 4 |
| NCBI Synonym Full Names: | enhancer of mRNA decapping 4 |
| NCBI Official Symbol: | EDC4 |
| NCBI Official Synonym Symbols: | GE1; Ge-1; RCD8; HEDL5; HEDLS; RCD-8 |
| NCBI Protein Information: | enhancer of mRNA-decapping protein 4; autoantigen Ge-1; autoantigen RCD-8; human enhancer of decapping large subunit |
| UniProt Protein Name: | Enhancer of mRNA-decapping protein 4 |
| UniProt Synonym Protein Names: | Autoantigen Ge-1; Autoantigen RCD-8; Human enhancer of decapping large subunit |
| Protein Family: | Enhancer of mRNA-decapping protein |
| UniProt Gene Name: | EDC4 |
| UniProt Entry Name: | EDC4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EDC4 antibody (CAB15418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
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