Anti-DOK5 Antibody (CAB13726)
- SKU:
- CAB13726
- Product type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-DOK5 Antibody |
| 抗体コード: | CAB13726 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 237-306 of human DOK5 (NP_060901.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Mouse, Rat |
| ポジティブサンプル: | Mouse kidney, Mouse liver, Rat skeletal muscle |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 237-306 of human DOK5 (NP_060901.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | LLQS VKNS MLQM KMSE RAAS LSTM VPLP RSAY WQHI TRQH STGQ LYRL QDVS SPLK LHRT ETFP AYRS EH |
| 遺伝子ID: | 55816 |
| Uniprot: | Q9P104 |
| セルラーロケーション: | |
| 計算された分子量: | 22kDa/35kDa |
| 観察された分子量: | 30kDa |
| 同義語: | DOK5, C20orf180, IRS-6, IRS6 |
| バックグラウンド: | The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. Unlike other DOK family proteins, this protein does not interact with RASGAP. This protein is up-regulated in patients with systemic sclerosis and is associated with fibrosis induced by insulin-like growth factor binding protein 5. Alternative splicing of this gene results in multiple transcript variants. |
| UniProt Protein Function: | DOK5: docking proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK5 functions in Ret-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of Ret in neuronal differentiation. Interacts with phosphorylated Ret. In contrast to other DOK proteins, it does not interact with RASGAP. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 20q13.2 Cellular Component: intracellular Molecular Function:insulin receptor binding; receptor signaling protein activity Biological Process: MAPKKK cascade; nervous system development; regulation of nerve growth factor receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway |
| NCBI Summary: | The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. Unlike other DOK family proteins, this protein does not interact with RASGAP. This protein is up-regulated in patients with systemic sclerosis and is associated with fibrosis induced by insulin-like growth factor binding protein 5. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jun 2014] |
| UniProt Code: | Q9P104 |
| NCBI GenInfo Identifier: | 26393190 |
| NCBI Gene ID: | 55816 |
| NCBI Accession: | Q9P104.2 |
| UniProt Secondary Accession: | Q9P104,Q5T7Y0, Q5TE53, Q8TEW7, Q96H13, Q9BZ24, Q9NQF4 Q9Y411, |
| UniProt Related Accession: | Q9P104 |
| Molecular Weight: | 22,824 Da |
| NCBI Full Name: | Docking protein 5 |
| NCBI Synonym Full Names: | docking protein 5 |
| NCBI Official Symbol: | DOK5 |
| NCBI Official Synonym Symbols: | IRS6; IRS-6; C20orf180 |
| NCBI Protein Information: | docking protein 5 |
| UniProt Protein Name: | Docking protein 5 |
| UniProt Synonym Protein Names: | Downstream of tyrosine kinase 5; Insulin receptor substrate 6; IRS-6; IRS6 |
| Protein Family: | Docking protein |
| UniProt Gene Name: | DOK5 |
| UniProt Entry Name: | DOK5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DOK5 antibody (CAB13726) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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