Anti-DOK4 Antibody (CAB0226)
- SKU:
- CAB0226
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-DOK4 Antibody |
| 抗体コード: | CAB0226 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 240-326 of human DOK4 (NP_060580.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | MCF7 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 240-326 of human DOK4 (NP_060580.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | EMEK NVRL LNKG TEHY SYPC TPTT MLPR SAYW HHIT GSQN IAEA SSYA GEGY GAAQ ASSE TDLL NRFI LLKP KPSQ GDSS EAKT PSQ |
| 遺伝子ID: | 55715 |
| Uniprot: | Q8TEW6 |
| セルラーロケーション: | |
| 計算された分子量: | 37kDa |
| 観察された分子量: | 37kDa |
| 同義語: | DOK4, IRS-5, IRS5 |
| バックグラウンド: | DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway (By similarity). Putative link with downstream effectors of RET in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells. |
| UniProt Protein Function: | DOK4: docking proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in Ret-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of Ret in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells. Interacts with Ret and TIE2. Interaction with Ret is mediated through the PTB domain and requires phosphorylation of Ret Y1062. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Cell development/differentiation Chromosomal Location of Human Ortholog: 16q21 Molecular Function:protein binding; receptor signaling protein activity; insulin receptor binding Biological Process: nervous system development; MAPKKK cascade; transmembrane receptor protein tyrosine kinase signaling pathway |
| UniProt Code: | Q8TEW6 |
| NCBI GenInfo Identifier: | 84029600 |
| NCBI Gene ID: | 55715 |
| NCBI Accession: | Q8TEW6.2 |
| UniProt Secondary Accession: | Q8TEW6,O75209, Q9BTP2, Q9NVV3, |
| UniProt Related Accession: | Q8TEW6 |
| Molecular Weight: | 326 |
| NCBI Full Name: | Docking protein 4 |
| NCBI Synonym Full Names: | docking protein 4 |
| NCBI Official Symbol: | DOK4 |
| NCBI Protein Information: | docking protein 4; IRS5; IRS-5; insulin receptor substrate 5; downstream of tyrosine kinase 4 |
| UniProt Protein Name: | Docking protein 4 |
| UniProt Synonym Protein Names: | Downstream of tyrosine kinase 4; Insulin receptor substrate 5; IRS-5; IRS5 |
| Protein Family: | Docking protein |
| UniProt Gene Name: | DOK4 |
| UniProt Entry Name: | DOK4_HUMAN |
 | Western blot analysis of extracts of MCF-7 cells, using DOK4 antibody (CAB0226) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded Human colon carcinoma using DOK4 Rabbit pAb (CAB0226) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using DOK4 antibody (CAB0226). Blue: DAPI for nuclear staining. |
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