Anti-DOCK2 Antibody (CAB3595)
- SKU:
- CAB3595
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-DOCK2 Antibody |
| 抗体コード: | CAB3595 |
| 抗体サイズ: | 20uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1551-1830 of human DOCK2 (NP_004937.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:1000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | Jurkat |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1551-1830 of human DOCK2 (NP_004937.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | FTEE YVRD HPED QDKL THLK DLIA WQIP FLGA GIKI HEKR VSDN LRPF HDRM EECF KNLK MKVE KEYG VREM PDFD DRRV GRPR SMLR SYRQ MSII SLAS MNSD CSTP SKPT SESF DLEL ASPK TPRV EQEE PISP GSTL PEVK LRRS KKRT KRSS VVFA DEKA AAES DLKR LSRK HEFM SDTN LSEH AAIP LKAS VLSQ MSFA SQSM PTIP ALAL SVAG IPGL DEAN TSPR LSQT FLQL SDGD KKTL TRKK VNQF FKTM LASK SAEE GKQI PDSL STDL |
| 遺伝子ID: | 1794 |
| Uniprot: | Q92608 |
| セルラーロケーション: | Cytoplasm, Endomembrane system, Peripheral membrane protein, cytoskeleton |
| 計算された分子量: | 38kDa/211kDa |
| 観察された分子量: | 240kDa |
| 同義語: | DOCK2, IMD40 |
| バックグラウンド: | The protein encoded by this gene belongs to the CDM protein family. It is specifically expressed in hematopoietic cells and is predominantly expressed in peripheral blood leukocytes. The protein is involved in remodeling of the actin cytoskeleton required for lymphocyte migration in response to chemokine signaling. It activates members of the Rho family of GTPases, for example RAC1 and RAC2, by acting as a guanine nucleotide exchange factor (GEF) to exchange bound GDP for free GTP. |
| UniProt Protein Function: | DOCK2: a hematopoietic cell-specific CDM family protein that is indispensable for lymphocyte chemotaxis. Plays a role in T cell receptor-induced activation of Rac2 and IL-2 transcription. Interacts with RAC, CRKL, and CD3-zeta. Association with ELMO1 is critical for DOCK2-mediated Rac activation. Two alternatively-spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; GAPs; GAPs, Rac/Rho; Adaptor/scaffold Chromosomal Location of Human Ortholog: 5q35.1 Cellular Component: cytoskeleton; membrane; endomembrane system; cytosol Molecular Function:protein binding; Rac guanyl-nucleotide exchange factor activity; T cell receptor binding Biological Process: alpha-beta T cell proliferation; viral reproduction; formation of immunological synapse; myeloid dendritic cell activation during immune response; small GTPase mediated signal transduction; positive regulation of phagocytosis; establishment of T cell polarity; lipid raft polarization; chemotaxis; actin cytoskeleton organization and biogenesis; negative thymic T cell selection; regulation of defense response to virus by virus; positive thymic T cell selection Disease: Immunodeficiency 40 |
| NCBI Summary: | The DOCK2 gene encodes a hematopoietic cell-specific CDM family protein that is indispensable for lymphocyte chemotaxis.[supplied by OMIM] |
| UniProt Code: | Q92608 |
| NCBI GenInfo Identifier: | 31377468 |
| NCBI Gene ID: | 1794 |
| NCBI Accession: | NP_004937.1 |
| UniProt Secondary Accession: | Q92608,Q2M3I0, Q96AK7, |
| UniProt Related Accession: | Q5XG91,Q6ZNS5,Q92608 |
| Molecular Weight: | 211,948 Da |
| NCBI Full Name: | dedicator of cytokinesis protein 2 |
| NCBI Synonym Full Names: | dedicator of cytokinesis 2 |
| NCBI Official Symbol: | DOCK2 |
| NCBI Official Synonym Symbols: | FLJ46592; KIAA0209 |
| NCBI Protein Information: | dedicator of cytokinesis protein 2; OTTHUMP00000160994; OTTHUMP00000223166; dedicator of cyto-kinesis 2 |
| UniProt Protein Name: | Dedicator of cytokinesis protein 2 |
| Protein Family: | Dedicator of cytokinesis protein |
| UniProt Gene Name: | DOCK2 |
| UniProt Entry Name: | DOCK2_HUMAN |
 | Western blot analysis of extracts of Jurkat cells, using DOCK2 antibody (CAB3595). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of L929 cells using DOCK2 Rabbit pAb (CAB3595) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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