Anti-Dnmt1 Antibody [KO Validated] (CAB19679)

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SKU:
CAB19679
Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
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Description

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抗体名:Anti-Dnmt1 Antibody [KO Validated]
抗体コード:CAB19679
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB IF
反応性:Human, Mouse, Rat
宿主種:Rabbit
免疫原:A synthesized peptide derived from human Dnmt1.
申し込み:WB IF
推奨希釈:WB 1:500 - 1:2000 IF 1:50 - 1:200
反応性:Human, Mouse, Rat
ポジティブサンプル:Jurkat, HeLa, 293T
免疫原:A synthesized peptide derived from human Dnmt1.
精製方法:Affinity purification
ストレージバッファ:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:Email for sequence
遺伝子ID:1786
Uniprot:P26358
セルラーロケーション:
計算された分子量:183kDa
観察された分子量:183KDa
Anti-Dnmt1 Antibody [KO Validated] (CAB19679)Western blot analysis of extracts from normal (control) and Dnmt1 knockout (KO) 293T cells, using Dnmt1 antibody (CAB19679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
Anti-Dnmt1 Antibody [KO Validated] (CAB19679)Western blot analysis of extracts of various cell lines, using Dnmt1 antibody (CAB19679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
Anti-Dnmt1 Antibody [KO Validated] (CAB19679)Immunofluorescence analysis of C6 cells using Dnmt1 antibody (CAB19679). Blue: DAPI for nuclear staining.
同義語:ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, m.HsaI, DNMT1
バックグラウンド:This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
UniProt Protein Function:DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described.
UniProt Protein Details:

Protein type:Methyltransferase, DNA; EC 2.1.1.37; Cell development/differentiation; Methyltransferase; Transcription regulation; Amino Acid Metabolism - cysteine and methionine

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; centric heterochromatin; replication fork; nucleus

Molecular Function:DNA (cytosine-5-)-methyltransferase activity; protein binding; methyl-CpG binding; DNA binding; zinc ion binding; RNA binding; chromatin binding; DNA-methyltransferase activity; transcription factor binding

Biological Process: negative regulation of histone H3-K9 methylation; transcription, DNA-dependent; maintenance of DNA methylation; negative regulation of gene expression, epigenetic; DNA methylation; gene expression; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; chromatin modification; gene silencing; regulation of gene expression, epigenetic; DNA methylation on cytosine; regulation of cell proliferation

Disease: Cerebellar Ataxia, Deafness, And Narcolepsy, Autosomal Dominant; Neuropathy, Hereditary Sensory, Type Ie

NCBI Summary:DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
UniProt Code:P26358
NCBI GenInfo Identifier:12231019
NCBI Gene ID:1786
NCBI Accession:P26358.2
UniProt Secondary Accession:P26358,Q9UHG5, Q9ULA2, Q9UMZ6, A0AV63, B7ZLW6,
UniProt Related Accession:P26358
Molecular Weight:1616
NCBI Full Name:DNA (cytosine-5)-methyltransferase 1
NCBI Synonym Full Names:DNA (cytosine-5-)-methyltransferase 1
NCBI Official Symbol:DNMT1  
NCBI Official Synonym Symbols:AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN  
NCBI Protein Information:DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; m.HsaI; DNA MTase HsaI; DNA methyltransferase HsaI; CXXC-type zinc finger protein 9
UniProt Protein Name:DNA (cytosine-5)-methyltransferase 1
UniProt Synonym Protein Names:CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT
UniProt Gene Name:DNMT1  
UniProt Entry Name:DNMT1_HUMAN
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