Anti-DNAJA1 Antibody (CAB11602)
- SKU:
- CAB11602
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-DNAJA1 Antibody |
| 抗体コード: | CAB11602 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 178-397 of human DNAJA1 (NP_001530.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | MCF7, HeLa, Jurkat, U-87MG, HepG2, Mouse brain, Mouse kidney, Mouse liver, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 178-397 of human DNAJA1 (NP_001530.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MECQ GHGE RISP KDRC KSCN GRKI VREK KILE VHID KGMK DGQK ITFH GEGD QEPG LEPG DIII VLDQ KDHA VFTR RGED LFMC MDIQ LVEA LCGF QKPI STLD NRTI VITS HPGQ IVKH GDIK CVLN EGMP IYRR PYEK GRLI IEFK VNFP ENGF LSPD KLSL LEKL LPER KEVE ETDE MDQV ELVD FDPN QERR RHYN GEAY EDDE HHPR GGVQ CQTS |
| 遺伝子ID: | 3301 |
| Uniprot: | P31689 |
| セルラーロケーション: | Cytoplasm, Lipid-anchor, Membrane, Microsome, Mitochondrion, Nucleus, perinuclear region |
| 計算された分子量: | 37kDa/44kDa |
| 観察された分子量: | 45kDa |
| 同義語: | DNAJA1, DJ-2, DjA1, HDJ2, HSDJ, HSJ-2, HSJ2, HSPF4, NEDD7, hDJ-2 |
| バックグラウンド: | This gene encodes a member of the DnaJ family of proteins, which act as heat shock protein 70 cochaperones. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. In humans, this gene has been implicated in positive regulation of virus replication through co-option by the influenza A virus. Several pseudogenes of this gene are found on other chromosomes. |
| UniProt Protein Function: | DNAJA1: Co-chaperone of Hsc70. Seems to play a role in protein import into mitochondria. |
| UniProt Protein Details: | Protein type:Chaperone; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 9p13.3 Cellular Component: membrane; mitochondrion; perinuclear region of cytoplasm; cytosol; nucleus; ubiquitin ligase complex Molecular Function:protein binding; G-protein-coupled receptor binding; low-density lipoprotein receptor binding; chaperone binding; metal ion binding; ubiquitin protein ligase binding; unfolded protein binding; Hsp70 protein binding; ATP binding Biological Process: negative regulation of JNK activity; protein folding; positive regulation of apoptosis; DNA damage response, detection of DNA damage; response to unfolded protein; regulation of protein transport; sperm motility; response to heat; androgen receptor signaling pathway; spermatogenesis; protein refolding; negative regulation of protein ubiquitination; negative regulation of apoptosis |
| UniProt Code: | P31689 |
| NCBI GenInfo Identifier: | 1706474 |
| NCBI Gene ID: | 3301 |
| NCBI Accession: | P31689.2 |
| UniProt Secondary Accession: | P31689,Q5T7Q0, Q86TL9, |
| UniProt Related Accession: | P31689 |
| Molecular Weight: | 397 |
| NCBI Full Name: | DnaJ homolog subfamily A member 1 |
| NCBI Synonym Full Names: | DnaJ (Hsp40) homolog, subfamily A, member 1 |
| NCBI Official Symbol: | DNAJA1 |
| NCBI Official Synonym Symbols: | DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSPF4; NEDD7; hDJ-2 |
| NCBI Protein Information: | dnaJ homolog subfamily A member 1; HSJ-2; human DnaJ protein 2; heat shock protein J2; dnaJ protein homolog 2; heat shock 40 kDa protein 4; heat shock protein, DNAJ-like 2; neural precursor cell expressed, developmentally down-regulated 7 |
| UniProt Protein Name: | DnaJ homolog subfamily A member 1 |
| UniProt Synonym Protein Names: | DnaJ protein homolog 2; HSDJ; Heat shock 40 kDa protein 4; Heat shock protein J2; HSJ-2; Human DnaJ protein 2; hDj-2 |
| UniProt Gene Name: | DNAJA1 |
| UniProt Entry Name: | DNJA1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DNAJA1 antibody (CAB11602) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of NIH/3T3 cells using DNAJA1 antibody (CAB11602) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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