Anti-DNAAF1 Antibody (CAB16593)
- SKU:
- CAB16593
- Product type:
- Antibody
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-DNAAF1 Antibody |
| 抗体コード: | CAB16593 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 516-725 of human DNAAF1 (NP_848547.4). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Rat |
| ポジティブサンプル: | Rat testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 516-725 of human DNAAF1 (NP_848547.4). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | VATE GVFV TELD GTRT EDLE TIRL ETKE TFCI DDLP DLED DDET GKSL EDQN MCFP KIEV ISSL SDDS DPEL DYTS LPVL ENLP TDTL SNIF AVSK DTSK AARV PFTD IFKK EAKR DLEI RKQD TKSP RPLI QELS DEDP SGQL LMPP TCQR DAAP LTSS GDRD SDFL AASS PVPT ESAA TPPE TCVG VAQP SQAL PTWD LTAF PAPK AS |
| 遺伝子ID: | 123872 |
| Uniprot: | Q8NEP3 |
| セルラーロケーション: | Cell projection, Cytoplasm, cilium, cytoskeleton, spindle pole |
| 計算された分子量: | 47kDa/53kDa/80kDa |
| 観察された分子量: | 80kDa |
| 同義語: | DNAAF1, CILD13, LRRC50, ODA7, swt |
| バックグラウンド: | The protein encoded by this gene is cilium-specific and is required for the stability of the ciliary architecture. It is involved in the regulation of microtubule-based cilia and actin-based brush border microvilli. Mutations in this gene are associated with primary ciliary dyskinesia-13. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | LRRC50: Cilium-specific protein required for the stability of the ciliary architecture. Plays a role in cytoplasmic preassembly of dynein arms. Involved in regulation of microtubule-based cilia and actin-based brush border microvilli. Defects in DNAAF1 are the cause of primary ciliary dyskinesia type 13 (CILD13). A disorder characterized by abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit randomization of left-right body asymmetry and situs inversus, due to dysfunction of monocilia at the embryonic node. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome. At ultrastructural level, CILD13 is characterized by a marked reduction or absence of both dynein arms from the cilia. Belongs to the DNAAF1 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 16q24.1 Cellular Component: spindle pole; cytoplasm; plasma membrane; axoneme; nucleus Molecular Function:dynein binding Biological Process: heart looping; lung development Disease: Ciliary Dyskinesia, Primary, 13 |
| NCBI Summary: | The protein encoded by this gene is cilium-specific and is required for the stability of the ciliary architecture. It is involved in the regulation of microtubule-based cilia and actin-based brush border microvilli. Mutations in this gene are associated with primary ciliary dyskinesia-13.[provided by RefSeq, May 2010] |
| UniProt Code: | Q8NEP3 |
| NCBI GenInfo Identifier: | 157674358 |
| NCBI Gene ID: | 123872 |
| NCBI Accession: | NP_848547.4 |
| UniProt Secondary Accession: | Q8NEP3,Q69YI8, Q69YJ0, Q69YW5, Q96LP3, Q96MB6, B4DJA3 |
| UniProt Related Accession: | Q8NEP3 |
| Molecular Weight: | 80,026 Da |
| NCBI Full Name: | dynein assembly factor 1, axonemal |
| NCBI Synonym Full Names: | dynein, axonemal, assembly factor 1 |
| NCBI Official Symbol: | DNAAF1 |
| NCBI Official Synonym Symbols: | ODA7; CILD13; LRRC50 |
| NCBI Protein Information: | dynein assembly factor 1, axonemal; leucine rich repeat containing 50; outer row dynein assembly 7 homolog; leucine-rich repeat-containing protein 50 |
| UniProt Protein Name: | Dynein assembly factor 1, axonemal |
| UniProt Synonym Protein Names: | Leucine-rich repeat-containing protein 50 |
| UniProt Gene Name: | DNAAF1 |
| UniProt Entry Name: | DAAF1_HUMAN |
 | Western blot analysis of extracts of rat testis, using DNAAF1 antibody (CAB16593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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