Anti-DIAPH3 Antibody (CAB10351)
- SKU:
- CAB10351
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-DIAPH3 Antibody |
| 抗体コード: | CAB10351 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 630-849 of human DIAPH3 (NP_112194.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | MCF7, HeLa, HT-1080, NCI-H460 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 630-849 of human DIAPH3 (NP_112194.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | KYPD ILNF VDDL EPLD KASK VSVE TLEK NLRQ MGRQ LQQL EKEL ETFP PPED LHDK FVTK MSRF VISA KEQY ETLS KLHE NMEK LYQS IIGY YAID VKKV SVED FLTD LNNF RTTF MQAI KENI KKRE AEEK EKRV RIAK ELAE RERL ERQQ KKKR LLEM KTEG DETG VMDN LLEA LQSG AAFR DRRK RTPM PKDV RQSL SPMS QRPV LKVC NHGN KPYL |
| 遺伝子ID: | 81624 |
| Uniprot: | Q9NSV4 |
| セルラーロケーション: | Cytoplasm, cytosol |
| 計算された分子量: | 79kDa/98kDa/127-136kDa |
| 観察された分子量: | 137kDa |
| 同義語: | DIAPH3, AN, AUNA1, DIA2, DRF3, NSDAN, diap3, mDia2 |
| バックグラウンド: | This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | Diaphanous-3: Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Defects in DIAPH3 are the cause of auditory neuropathy, autosomal dominant, type 1 (AUNA1). A form of sensorineural hearing loss with absent or severely abnormal auditory brainstem response, in the presence of normal cochlear outer hair cell function and normal otoacoustic emissions. Auditory neuropathies result from a lesion in the area including the inner hair cells, connections between the inner hair cells and the cochlear branch of the auditory nerve, the auditory nerve itself and auditory pathways of the brainstem. A disease- causing mutation in the conserved 5'-UTR leads to increased protein expression (PubMed:20624953). Belongs to the formin homology family. Diaphanous subfamily. 7 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 13q21.2 Cellular Component: cytosol; nucleus Molecular Function:Rho GTPase binding; actin binding Biological Process: spermatogenesis; actin cytoskeleton organization and biogenesis Disease: Auditory Neuropathy, Autosomal Dominant, 1 |
| NCBI Summary: | This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] |
| UniProt Code: | Q9NSV4 |
| NCBI GenInfo Identifier: | 158520000 |
| NCBI Gene ID: | 81624 |
| NCBI Accession: | Q9NSV4.4 |
| UniProt Related Accession: | Q9NSV4 |
| Molecular Weight: | |
| NCBI Full Name: | Protein diaphanous homolog 3 |
| NCBI Synonym Full Names: | diaphanous related formin 3 |
| NCBI Official Symbol: | DIAPH3 |
| NCBI Official Synonym Symbols: | AN; DIA2; DRF3; AUNA1; NSDAN; diap3; mDia2 |
| NCBI Protein Information: | protein diaphanous homolog 3 |
| UniProt Protein Name: | Protein diaphanous homolog 3 |
| UniProt Synonym Protein Names: | Diaphanous-related formin-3; DRF3; MDia2 |
| Protein Family: | Protein diaphanous |
| UniProt Gene Name: | DIAPH3 |
| UniProt Entry Name: | DIAP3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DIAPH3 antibody (CAB10351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
View AllClose