Anti-DHX58 Antibody (CAB8257)
- SKU:
- CAB8257
- Product type:
- Antibody
- Application:
- WB
- Application:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | DHX58 Rabbit Polyclonal Antibody |
| 抗体コード: | CAB8257 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 389-678 of human DHX58 (NP_077024.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse liver, Mouse brain, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 389-678 of human DHX58 (NP_077024.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | QQQG LQTV DIRA QLLI GAGN SSQS THMT QRDQ QEVI QKFQ DGTL NLLV ATSV AEEG LDIP HCNV VVRY GLLT NEIS MVQA RGRA RADQ SVYA FVAT EGSR ELKR ELIN EALE TLME QAVA AVQK MDQA EYQA KIRD LQQA ALTK RAAQ AAQR ENQR QQFP VEHV QLLC INCM VAVG HGSD LRKV EGTH HVNV NPNF SNYY NVSR DPVV INKV FKDW KPGG VISC RNCG EVWG LQMI YKSV KLPV LKVR SMLL ETPQ GRIQ AKKW SRVP FSVP DFDF LQHC AENL SDLS LD |
| 遺伝子ID: | 79132 |
| Uniprot: | Q96C10 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 76kDa |
| 観察された分子量: | 77kDa |
| 同義語: | DHX58, D11LGP2, D11lgp2e, LGP2, RLR-3 |
| バックグラウンド: |
| UniProt Protein Function: | DHX58: Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1- dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate. Belongs to the helicase family. RLR subfamily. |
| UniProt Protein Details: | Protein type:Helicase; Inhibitor; EC 3.6.4.13 Chromosomal Location of Human Ortholog: 17q21.2 Cellular Component: cytoplasm Molecular Function:ATP binding; DNA binding; double-stranded RNA binding; helicase activity; protein binding; single-stranded RNA binding; zinc ion binding Biological Process: defense response to virus; innate immune response; metabolic process; negative regulation of innate immune response; negative regulation of interferon type I production; positive regulation of interferon type I production; regulation of innate immune response; response to virus; viral reproduction |
| UniProt Code: | Q96C10 |
| NCBI GenInfo Identifier: | 50401123 |
| NCBI Gene ID: | 79132 |
| NCBI Accession: | Q96C10.1 |
| UniProt Secondary Accession: | Q96C10,Q9HAM6, |
| UniProt Related Accession: | Q96C10 |
| Molecular Weight: | 76,613 Da |
| NCBI Full Name: | Probable ATP-dependent RNA helicase DHX58 |
| NCBI Synonym Full Names: | DEXH-box helicase 58 |
| NCBI Official Symbol: | DHX58 |
| NCBI Official Synonym Symbols: | LGP2; RLR-3; D11LGP2; D11lgp2e |
| NCBI Protein Information: | probable ATP-dependent RNA helicase DHX58 |
| UniProt Protein Name: | Probable ATP-dependent RNA helicase DHX58 |
| UniProt Synonym Protein Names: | Probable ATP-dependent helicase LGP2; Protein D11Lgp2 homolog; RIG-I-like receptor 3; RLR-3; RIG-I-like receptor LGP2; RLR |
| UniProt Gene Name: | DHX58 |
| UniProt Entry Name: | DHX58_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DHX58 antibody (CAB8257) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of U2OS cells using DHX58 antibody (CAB8257) at dilution of 1:100. Blue: DAPI for nuclear staining. |