Anti-DGCR8 Antibody (CAB18630)
- SKU:
- CAB18630
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-DGCR8 Antibody |
| 抗体コード: | CAB18630 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IP |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant protein of human DGCR8. |
| 申し込み: | WB IHC IP |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HepG2, HeLa, 293T, Mouse testis |
| 免疫原: | Recombinant protein of human DGCR8. |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 54487 |
| Uniprot: | Q8WYQ5 |
| セルラーロケーション: | Nucleus, nucleolus |
| 計算された分子量: | 32kDa/82kDa/86kDa |
| 観察された分子量: | 110kDa |
 | Western blot - DGCR8 Rabbit pAb (CAB18630) |
| 同義語: | DGCR8, C22orf12, DGCRK6, Gy1, pasha, microprocessor complex subunit DGCR8 |
| バックグラウンド: | This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. |
| UniProt Protein Function: | DGCR8: a double strand RNA binding protein (dsRBP) which is essential for the first processing step of primary miRNAs (pri-miRNAs). Apparently binds and is activated by heme. A nuclear complex of Drosha with DGCR8 cleaves pri-miRNA substrates into 60-70 nucleotide intermediates (pre-miRNA), thus initiating the microRNA maturation process. Drosha provides the catalytic site for this cleavage, whereas DGCR8 (Pasha) anchors substrate pri-miRNAs. Pre-miRNAs are then processed by the cytoplasmic Dicer-TRBP complex to approximately 20-22 nt mature miRNAs. |
| UniProt Protein Details: | Protein type:RNA processing; RNA-binding; Nucleolus Chromosomal Location of Human Ortholog: 22q11.2 Cellular Component: cytoplasm; microtubule cytoskeleton; nucleoplasm; nucleus Molecular Function:double-stranded RNA binding; heme binding; protein binding; protein homodimerization activity; ribonuclease III activity Biological Process: primary microRNA processing; RNA-mediated gene silencing Disease: Velocardiofacial Syndrome |
| NCBI Summary: | This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010] |
| UniProt Code: | Q8WYQ5 |
| NCBI GenInfo Identifier: | 23813990 |
| NCBI Gene ID: | 54487 |
| NCBI Accession: | Q8WYQ5.1 |
| UniProt Secondary Accession: | Q8WYQ5,Q6DCB2, Q6MZE9, Q6Y2L0, Q96G39, Q96GP8, Q9H6L8 Q9H6T7, Q9NRW2, B2R8G1, |
| UniProt Related Accession: | Q8WYQ5 |
| Molecular Weight: | 82,750 Da |
| NCBI Full Name: | Microprocessor complex subunit DGCR8 |
| NCBI Synonym Full Names: | DGCR8 microprocessor complex subunit |
| NCBI Official Symbol: | DGCR8 |
| NCBI Official Synonym Symbols: | Gy1; pasha; DGCRK6; C22orf12 |
| NCBI Protein Information: | microprocessor complex subunit DGCR8 |
| UniProt Protein Name: | Microprocessor complex subunit DGCR8 |
| UniProt Synonym Protein Names: | DiGeorge syndrome critical region 8 |
| Protein Family: | Microprocessor complex |
| UniProt Gene Name: | DGCR8 |
| UniProt Entry Name: | DGCR8_HUMAN |