Anti-DGAT1 Antibody (CAB6857)
- SKU:
- CAB6857
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-DGAT1 Antibody |
| 抗体コード: | CAB6857 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human DGAT1 (NP_036211.2). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HL-60 |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human DGAT1 (NP_036211.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | AHTI LFLK LFSY RDVN SWCR RARA KAAS AGKK ASSA AAPH TVSY PDNL TYRD LYYF LFAP TLCY ELNF PRSP RIRK RFLL RRIL EMLF FTQL QVGL IQQW M |
| 遺伝子ID: | 8694 |
| Uniprot: | O75907 |
| セルラーロケーション: | Endoplasmic reticulum membrane, Multi-pass membrane protein |
| 計算された分子量: | 55kDa |
| 観察された分子量: | 54kDa |
| 同義語: | DGAT1, ARAT, ARGP1, DGAT, DIAR7 |
| バックグラウンド: | This gene encodes an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases. |
| UniProt Protein Function: | DGAT1: Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders. Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily. |
| UniProt Protein Details: | Protein type:Transferase; Cofactor and Vitamin Metabolism - retinol; EC 2.3.1.76; Lipid Metabolism - glycerolipid; Membrane protein, multi-pass; EC 2.3.1.20; Membrane protein, integral Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: endoplasmic reticulum membrane; integral to membrane Molecular Function:2-acylglycerol O-acyltransferase activity; protein binding; transferase activity, transferring acyl groups; retinol O-fatty-acyltransferase activity; diacylglycerol O-acyltransferase activity Biological Process: sequestering of lipid; triacylglycerol metabolic process; retinol metabolic process; phospholipid metabolic process; fatty acid homeostasis; glycerophospholipid biosynthetic process; triacylglycerol biosynthetic process; diacylglycerol metabolic process; cellular lipid metabolic process Disease: Diarrhea 7 |
| NCBI Summary: | This gene encodes an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases. [provided by RefSeq, Jul 2013] |
| UniProt Code: | O75907 |
| NCBI GenInfo Identifier: | 33302597 |
| NCBI Gene ID: | 8694 |
| NCBI Accession: | O75907.2 |
| UniProt Secondary Accession: | O75907,Q96BB8, B2RWQ2, D3DWL6, |
| UniProt Related Accession: | O75907 |
| Molecular Weight: | 488 |
| NCBI Full Name: | Diacylglycerol O-acyltransferase 1 |
| NCBI Synonym Full Names: | diacylglycerol O-acyltransferase 1 |
| NCBI Official Symbol: | DGAT1 |
| NCBI Official Synonym Symbols: | ARAT; DGAT; ARGP1; DIAR7 |
| NCBI Protein Information: | diacylglycerol O-acyltransferase 1; ACAT related gene product 1; diglyceride acyltransferase; acyl-CoA:diacylglycerol acyltransferase; acyl-CoA retinol O-fatty-acyltransferase; acyl coenzyme A:cholesterol acyltransferase related gene 1 |
| UniProt Protein Name: | Diacylglycerol O-acyltransferase 1 |
| UniProt Synonym Protein Names: | ACAT-related gene product 1; Acyl-CoA retinol O-fatty-acyltransferase (EC:2.3.1.76); ARAT; Retinol O-fatty-acyltransferase; Diglyceride acyltransferase |
| Protein Family: | Diacylglycerol O-acyltransferase |
| UniProt Gene Name: | DGAT1 |
| UniProt Entry Name: | DGAT1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DGAT1 antibody (CAB6857) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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