Anti-DDX50 Antibody (CAB8628)
- SKU:
- CAB8628
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-DDX50 Antibody |
| 抗体コード: | CAB8628 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human DDX50 (NP_076950.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | A-431, Jurkat, HepG2 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human DDX50 (NP_076950.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MPGK LLWG DIME LEAP LEES ESQK KERQ KSDR RKSR HHYD SDEK SETR ENGV TDDL DAPK AKKS KMKE KLNG DTEE GFNR LSDE FSKS HKSR RKDL PNGD IDEY EKKS KRVS SLDT STHK SSDN KLEE TLTR EQK |
| 遺伝子ID: | 79009 |
| Uniprot: | Q9BQ39 |
| セルラーロケーション: | Nucleus, nucleolus |
| 計算された分子量: | 82kDa |
| 観察された分子量: | 105kDa |
| 同義語: | DDX50, GU2, GUB, RH-II/GuB, mcdrh |
| バックグラウンド: | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined. |
| UniProt Protein Function: | DDX50: an RNA helicase of the DEAD-box family. |
| UniProt Protein Details: | Protein type:EC 3.6.4.13; RNA-binding; Helicase; Nucleolus Chromosomal Location of Human Ortholog: 10q22.1 Cellular Component: membrane; nucleolus; plasma membrane Molecular Function:helicase activity; ATP binding Biological Process: metabolic process |
| NCBI Summary: | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9BQ39 |
| NCBI GenInfo Identifier: | 13129006 |
| NCBI Gene ID: | 79009 |
| NCBI Accession: | NP_076950.1 |
| UniProt Secondary Accession: | Q9BQ39,Q99MJ9, |
| UniProt Related Accession: | Q9BQ39 |
| Molecular Weight: | 82,565 Da |
| NCBI Full Name: | ATP-dependent RNA helicase DDX50 |
| NCBI Synonym Full Names: | DEAD (Asp-Glu-Ala-Asp) box polypeptide 50 |
| NCBI Official Symbol: | DDX50 |
| NCBI Official Synonym Symbols: | GU2; GUB; mcdrh; RH-II/GuB |
| NCBI Protein Information: | ATP-dependent RNA helicase DDX50; gu-beta; DEAD box protein 50; nucleolar protein GU2; RNA helicase II/Gu beta; malignant cell derived RNA helicase |
| UniProt Protein Name: | ATP-dependent RNA helicase DDX50 |
| UniProt Synonym Protein Names: | DEAD box protein 50; Gu-beta; Nucleolar protein Gu2 |
| Protein Family: | ATP-dependent RNA helicase |
| UniProt Gene Name: | DDX50 |
| UniProt Entry Name: | DDX50_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DDX50 antibody (CAB8628) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse testis using DDX50 antibody (CAB8628) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using DDX50 antibody (CAB8628) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using DDX50 antibody (CAB8628) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using DDX50 antibody (CAB8628) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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