Anti-DDX3Y Antibody (CAB14810)
- SKU:
- CAB14810
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-DDX3Y Antibody |
| 抗体コード: | CAB14810 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DDX3Y (NP_001116137.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | A-549, HeLa, Rat testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DDX3Y (NP_001116137.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MSHV VVKN DPEL DQQL ANLD LNSE KQSG GAST ASKG RYIP PHLR NREA SKGF HDKD SSGW SCSK DKDA YSSF GSRD SRGK PGYF SERG SGSR GRFD DRGR SDYD GIGN RERP GFGR FERS GHSR WCDK SVED DWSK PLPP SERL EQEL FSGG NTGI NFEK YDDI PVEA TGSN CPPH IENF SDID MGEI IMGN IELT RYTR PTPV QKHA IP |
| 遺伝子ID: | 8653 |
| Uniprot: | O15523 |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 32kDa/72kDa/73kDa |
| 観察された分子量: | 73kDa |
| 同義語: | DDX3Y, DBY |
| バックグラウンド: | The protein encoded by this gene is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif. These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions. This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of this gene is not complemented by DDX3X. Mutations in this gene result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome. Pseudogenes sharing similarity to both this gene and the DDX3X paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | DDX3Y: a Y-linked DEAD box protein. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. DBY mutations causes male infertility, Sertoli cell-only syndrome or severe hypospermatogenesis, suggesting that this gene plays a key role in the spermatogenic process. Two alternatively spliced transcripts differ only in the length of the 3' UTR. |
| UniProt Protein Details: | Protein type:RNA-binding; EC 3.6.4.13; Helicase Chromosomal Location of Human Ortholog: Yq11 Cellular Component: cytoplasm; membrane Molecular Function:ATP-dependent RNA helicase activity Biological Process: chromosome segregation; regulation of gene expression; RNA secondary structure unwinding; translational initiation Disease: Spermatogenic Failure, Y-linked, 2 |
| NCBI Summary: | The protein encoded by this gene is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif. These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions. This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of this gene is not complemented by DDX3X. Mutations in this gene result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome. Pseudogenes sharing similarity to both this gene and the DDX3X paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014] |
| UniProt Code: | O15523 |
| NCBI GenInfo Identifier: | 73620957 |
| NCBI Gene ID: | 8653 |
| NCBI Accession: | O15523.2 |
| UniProt Secondary Accession: | O15523,Q8IYV7, B4DK29, B4DXX7, |
| UniProt Related Accession: | O15523 |
| Molecular Weight: | 32,416 Da |
| NCBI Full Name: | ATP-dependent RNA helicase DDX3Y |
| NCBI Synonym Full Names: | DEAD-box helicase 3, Y-linked |
| NCBI Official Symbol: | DDX3Y |
| NCBI Official Synonym Symbols: | DBY |
| NCBI Protein Information: | ATP-dependent RNA helicase DDX3Y |
| UniProt Protein Name: | ATP-dependent RNA helicase DDX3Y |
| UniProt Synonym Protein Names: | DEAD box protein 3, Y-chromosomal |
| Protein Family: | ATP-dependent RNA helicase |
| UniProt Gene Name: | DDX3Y |
| UniProt Entry Name: | DDX3Y_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DDX3Y antibody (CAB14810) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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