Anti-DDX39B 抗体 (CAB8356)

抗体名:	Anti-DDX39B Antibody
抗体コード:	CAB8356
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IF
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 319-428 of human DDX39B (NP_004631.1).

申し込み:	WB IHC IF
推奨希釈:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
反応性:	Human, Mouse, Rat
ポジティブサンプル:	293T, HeLa, LO2, SKOV3, K-562, SW620

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 319-428 of human DDX39B (NP_004631.1).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	RGMP QEER LSRY QQFK DFQR RILV ATNL FGRG MDIE RVNI AFNY DMPE DSDT YLHR VARA GRFG TKGL AITF VSDE NDAK ILND VQDR FEVN ISEL PDEI DISS YIEQ TR
遺伝子ID:	7919
Uniprot:	Q13838
セルラーロケーション:	Cytoplasm, Nucleus, Nucleus speckle
計算された分子量:	48kDa/50kDa
観察された分子量:	49kDa

同義語:

DDX39B, BAT1, D6S81E, UAP56

バックグラウンド:

This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and it also plays an important role in mRNA export from the nucleus to the cytoplasm. This gene belongs to a cluster of genes localized in the vicinity of the genes encoding tumor necrosis factor alpha and tumor necrosis factor beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on both chromosomes 6 and 11. Read-through transcription also occurs between this gene and the upstream ATP6V1G2 (ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2) gene.

UniProt Protein Function:	BAT1: Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4. Belongs to the DEAD box helicase family. DECD subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Spliceosome; EC 3.6.4.13; RNA splicing; Helicase; RNA-binding Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: cytoplasm; nucleoplasm; nucleus; spliceosome Molecular Function:ATP-dependent protein binding; ATP-dependent RNA helicase activity; ATPase activity; protein binding; RNA-dependent ATPase activity; U4 snRNA binding; U6 snRNA binding Biological Process: intronless viral mRNA export from host nucleus; mRNA 3'-end processing; mRNA export from nucleus; nuclear mRNA splicing, via spliceosome; positive regulation of RNA elongation; RNA export from nucleus; RNA secondary structure unwinding; RNA splicing; spliceosome assembly; termination of RNA polymerase II transcription
NCBI Summary:	This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and it also plays an important role in mRNA export from the nucleus to the cytoplasm. This gene belongs to a cluster of genes localized in the vicinity of the genes encoding tumor necrosis factor alpha and tumor necrosis factor beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on both chromosomes 6 and 11. Read-through transcription also occurs between this gene and the upstream ATP6V1G2 (ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2) gene. [provided by RefSeq, Feb 2011]
UniProt Code:	Q13838
NCBI GenInfo Identifier:	2500529
NCBI Gene ID:	7919
NCBI Accession:	Q13838.1
UniProt Secondary Accession:	Q13838,O43496, Q0EFA1, Q2L6F9, Q53GL9, Q5RJ64, Q5RJ66 Q5ST94, Q5STB4, Q5STB5, Q5STB7, B0S8C0,
UniProt Related Accession:	Q13838
Molecular Weight:	50,679 Da
NCBI Full Name:	Spliceosome RNA helicase DDX39B
NCBI Synonym Full Names:	DEAD-box helicase 39B
NCBI Official Symbol:	DDX39B
NCBI Official Synonym Symbols:	BAT1; UAP56; D6S81E
NCBI Protein Information:	spliceosome RNA helicase DDX39B
UniProt Protein Name:	Spliceosome RNA helicase DDX39B
UniProt Synonym Protein Names:	56 kDa U2AF65-associated protein; ATP-dependent RNA helicase p47; DEAD box protein UAP56; HLA-B-associated transcript 1 protein
Protein Family:	Spliceosome RNA helicase
UniProt Gene Name:	DDX39B
UniProt Entry Name:	DX39B_HUMAN

	Western blot analysis of extracts of various cell lines, using DDX39B antibody (CAB8356) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat liver using DDX39B antibody (CAB8356) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat ovary using DDX39B antibody (CAB8356) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using DDX39B antibody (CAB8356) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat spleen using DDX39B antibody (CAB8356) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)