Anti-DDX31 Antibody (CAB15892)
- SKU:
- CAB15892
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-DDX31 Antibody |
| 抗体コード: | CAB15892 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 652-851 of human DDX31 (NP_073616.6). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | A-549 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 652-851 of human DDX31 (NP_073616.6). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | EIKM EDIL CVLT RDDC FKGK RWGA QKSH AVGP QEIR ERAT VLQT VFED YVHS SERR VSWA KKAL QSFI QAYA TYPR ELKH IFHV RSLH LGHV AKSF GLRD APRN LSAL TRKK RKAH VKRP DLHK KTQS KHSL AEIL RSEY SSGM EADI AKVK KQNA PGEP GGRP LQHS LQPT PCFG RGKT LKWR KTQK GVQR DSKT SQKV |
| 遺伝子ID: | 64794 |
| Uniprot: | Q9H8H2 |
| セルラーロケーション: | Nucleus, nucleolus |
| 計算された分子量: | 34kDa/64kDa/85kDa/94kDa |
| 観察された分子量: | 94kDa |
| 同義語: | DDX31, PPP1R25 |
| バックグラウンド: | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | DDX31: Probable ATP-dependent RNA helicase. Belongs to the DEAD box helicase family. DDX31/DBP7 subfamily. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.6.4.13; Hydrolase; Nucleolus Chromosomal Location of Human Ortholog: 9q34.13 Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; nucleolus Molecular Function:ATP binding; ATP-dependent RNA helicase activity; protein binding Biological Process: ribosome biogenesis and assembly; RNA secondary structure unwinding |
| NCBI Summary: | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9H8H2 |
| NCBI GenInfo Identifier: | 17505907 |
| NCBI Gene ID: | 64794 |
| NCBI Accession: | NP_073616.6 |
| UniProt Secondary Accession: | Q9H8H2,Q5K6N2, Q5K6N3, Q5K6N4, Q5VZJ4, Q5VZJ9, Q96E91 Q96NY2, Q96SX5, Q9H5K6, |
| UniProt Related Accession: | Q9H8H2 |
| Molecular Weight: | 64,137 Da |
| NCBI Full Name: | probable ATP-dependent RNA helicase DDX31 isoform 1 |
| NCBI Synonym Full Names: | DEAD-box helicase 31 |
| NCBI Official Symbol: | DDX31 |
| NCBI Official Synonym Symbols: | PPP1R25 |
| NCBI Protein Information: | probable ATP-dependent RNA helicase DDX31 |
| UniProt Protein Name: | Probable ATP-dependent RNA helicase DDX31 |
| UniProt Synonym Protein Names: | DEAD box protein 31; Helicain |
| Protein Family: | Probable ATP-dependent RNA helicase |
| UniProt Gene Name: | DDX31 |
| UniProt Entry Name: | DDX31_HUMAN |
 | Western blot analysis of extracts of A-549 cells, using DDX31 antibody (CAB15892) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min. |
 | Immunofluorescence analysis of HeLa cells using DDX31 antibody (CAB15892) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using DDX31 antibody (CAB15892) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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