Anti-DDDDK-Tag Antibody (CABE063)
- SKU:
- CABE063
- Product type:
- Antibody
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
Frequently bought together:
Description
| 抗体名: | Anti-DDDDK-Tag Antibody |
| 抗体コード: | CABE063 |
| 抗体サイズ: | 100 uL |
| 申し込み: | WB IHC IF IP |
| 反応性: | |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to DDDDK tag. |
| 申し込み: | WB IHC IF IP |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:500 IF 1:50 - 1:500 IP 1:50 - 1:500 |
| 反応性: | |
| ポジティブサンプル: | 293T |
| 免疫原: | A synthetic peptide corresponding to DDDDK tag. |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | DYKD DDDK |
| 遺伝子ID: | |
| Uniprot: | |
| セルラーロケーション: | |
| 計算された分子量: | |
| 観察された分子量: | Refer to Figures |
 | Western blot analysis of extracts of normal 293T cells and 293T transfected with Flag-GSK3B protein, using DDDDK-Tag antibody (CABE063) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of GFP-DDDDK transgenic HeLa cells using DDDDK-Tag antibody (CABE063). Green: GFP expression. Blue: DAPI for nuclear staining.Æ’€š |
 | Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells incubated using DDDDK-tag antibody (CABE063). A mock served as negative control and over-expressed 293T cell lysate served as positive control. |
| 同義語: | DDDDK, DDDDK tag, DDDDK-tag |
| バックグラウンド: | FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting. |