Anti-CYTH1 Antibody (CAB15351)
- SKU:
- CAB15351
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-CYTH1 Antibody |
| 抗体コード: | CAB15351 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human CYTH1 (NP_004753.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Raji, Mouse brain, Mouse heart, Mouse spleen, Rat heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human CYTH1 (NP_004753.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MEED DSYV PSDL TAEE RQEL ENIR RRKQ ELLA DIQR LKDE IAEV ANEI ENLG STEE RKNM QRNK QVAM GR |
| 遺伝子ID: | 9267 |
| Uniprot: | Q15438 |
| セルラーロケーション: | Cell membrane, Cytoplasm, Peripheral membrane protein, cytosol |
| 計算された分子量: | 39kDa/46kDa |
| 観察された分子量: | 46kDa |
| 同義語: | CYTH1, B2-1, CYTOHESIN-1, D17S811E, PSCD1, SEC7 |
| バックグラウンド: | The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This gene is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. A pseudogene of this gene has been defined on the X chromosome. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | cytohesin 1: Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF through replacement of GDP with GTP. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; GEFs; GEFs, ARF Chromosomal Location of Human Ortholog: 17q25 Cellular Component: extrinsic to internal side of plasma membrane; tight junction; cytoplasm; plasma membrane; trans-Golgi network; cytosol Molecular Function:protein binding; lipid binding; ARF guanyl-nucleotide exchange factor activity Biological Process: vesicle-mediated transport; regulation of cell adhesion; regulation of ARF protein signal transduction; positive regulation of GTPase activity |
| NCBI Summary: | The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This gene is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. A pseudogene of this gene has been defined on the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| UniProt Code: | Q15438 |
| NCBI GenInfo Identifier: | 2498175 |
| NCBI Gene ID: | 9267 |
| NCBI Accession: | Q15438.1 |
| UniProt Secondary Accession: | Q15438,Q9P123, Q9P124, A6NFW7, B7Z1T4, |
| UniProt Related Accession: | Q15438 |
| Molecular Weight: | 39,470 Da |
| NCBI Full Name: | Cytohesin-1 |
| NCBI Synonym Full Names: | cytohesin 1 |
| NCBI Official Symbol: | CYTH1 |
| NCBI Official Synonym Symbols: | B2-1; SEC7; PSCD1; D17S811E; CYTOHESIN-1 |
| NCBI Protein Information: | cytohesin-1; PH, SEC7 and coiled-coil domain-containing protein 1; SEC7 homolog B2-1; cytoadhesin 1; homolog of secretory protein SEC7; pleckstrin homology, Sec7 and coiled-coil domains 1 |
| UniProt Protein Name: | Cytohesin-1 |
| UniProt Synonym Protein Names: | PH, SEC7 and coiled-coil domain-containing protein 1; SEC7 homolog B2-1 |
| Protein Family: | Cytohesin |
| UniProt Gene Name: | CYTH1 |
| UniProt Entry Name: | CYH1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CYTH1 antibody (CAB15351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of L929 cells using CYTH1 antibody (CAB15351) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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