Anti-Cyclin B2 Antibody (CAB3351)
- SKU:
- CAB3351
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-Cyclin B2 Antibody |
| 抗体コード: | CAB3351 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Cyclin B2 (NP_004692.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, SW480, DU145, A-431, K-562, Mouse thymus |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Cyclin B2 (NP_004692.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MALL RRPT VSSD LENI DTGV NSKV KSHV TIRR TVLE EIGN RVTT RAAQ VAKK AQNT KVPV QPTK TTNV NKQL KPTA SVKP VQME KLAP KGPS PTPE DVSM |
| 遺伝子ID: | 9133 |
| Uniprot: | O95067 |
| セルラーロケーション: | |
| 計算された分子量: | 45kDa |
| 観察された分子量: | 45kDa |
| 同義語: | CCNB2, HsT17299, cyclin B2 |
| バックグラウンド: | Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. |
| UniProt Protein Function: | CCNB2: Essential for the control of the cell cycle at the G2/M (mitosis) transition. Belongs to the cyclin family. Cyclin AB subfamily. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 15q22.2 Cellular Component: microtubule cytoskeleton; nucleoplasm; centrosome; membrane; cytosol Molecular Function:protein binding; protein kinase binding Biological Process: mitosis; thymus development; cell division; regulation of cell cycle; in utero embryonic development; mitotic nuclear envelope disassembly; T cell homeostasis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; growth |
| NCBI Summary: | Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O95067 |
| NCBI GenInfo Identifier: | 5921731 |
| NCBI Gene ID: | 9133 |
| NCBI Accession: | O95067.1 |
| UniProt Related Accession: | O95067 |
| Molecular Weight: | ~ 45kDa |
| NCBI Full Name: | G2/mitotic-specific cyclin-B2 |
| NCBI Synonym Full Names: | cyclin B2 |
| NCBI Official Symbol: | CCNB2 |
| NCBI Official Synonym Symbols: | HsT17299 |
| NCBI Protein Information: | G2/mitotic-specific cyclin-B2 |
| UniProt Protein Name: | G2/mitotic-specific cyclin-B2 |
| Protein Family: | Light-harvesting protein |
| UniProt Gene Name: | CCNB2 |
| UniProt Entry Name: | CCNB2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Cyclin B2 antibody (CAB3351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |