Anti-CRYBB1 Antibody (CAB2590)
- SKU:
- CAB2590
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-CRYBB1 Antibody |
| 抗体コード: | CAB2590 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human CRYBB1 (NP_001878.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | MCF7, BT474 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human CRYBB1 (NP_001878.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MSQA AKAS ASAT VAVN PGPD TKGK GAPP AGTS PSPG TTLA PTTV PITS AKAA ELPP GNYR LVVF ELEN FQGR RAEF SGEC SNLA DRGF DRVR SIIV SAGP WVAF EQSN FRGE MFIL EKGE YPRW NTWS SSYR SDRL MSFR PIKM DAQE HKIS LFEG ANFK GNTI EIQG DDAP SLWV YGFS DRVG SVKV SSGT WVGY QYPG YRGY QYLL EPGD FRHW NEWG AFQP QMQS LRRL RDKQ WHLE GSFP VLAT EPPK |
| 遺伝子ID: | 1414 |
| Uniprot: | P53674 |
| セルラーロケーション: | |
| 計算された分子量: | 28kDa |
| 観察された分子量: | 36kDa |
| 同義語: | CRYBB1, CATCN3, CTRCT17 |
| バックグラウンド: | Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta basic group member, undergoes extensive cleavage at its N-terminal extension during lens maturation. It is also a member of a gene cluster with beta-A4, beta-B2, and beta-B3. |
| UniProt Protein Function: | CRYBB1: a major structural protein of the eye lens. A member of the beta/gamma-crystallin family. Specific cleavages in the N-terminal arm occur during lens maturation and give rise to truncated forms, leading to impaired oligomerization and protein insolubilization. The protease responsible for this partial degradation could be calpain II. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 22q12.1 Molecular Function:structural constituent of eye lens Biological Process: visual perception Disease: Cataract 17, Multiple Types |
| NCBI Summary: | Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta basic group member, undergoes extensive cleavage at its N-terminal extension during lens maturation. It is also a member of a gene cluster with beta-A4, beta-B2, and beta-B3. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P53674 |
| NCBI GenInfo Identifier: | 1706116 |
| NCBI Gene ID: | 1414 |
| NCBI Accession: | P53674.2 |
| UniProt Secondary Accession: | P53674,Q9WVJ5, |
| UniProt Related Accession: | P53674 |
| Molecular Weight: | 252 |
| NCBI Full Name: | Beta-crystallin B1 |
| NCBI Synonym Full Names: | crystallin, beta B1 |
| NCBI Official Symbol: | CRYBB1 |
| NCBI Official Synonym Symbols: | CATCN3; CTRCT17 |
| NCBI Protein Information: | beta-crystallin B1; beta-B1 crystallin; eye lens structural protein |
| UniProt Protein Name: | Beta-crystallin B1 |
| UniProt Synonym Protein Names: | Beta-B1 crystallin |
| Protein Family: | Beta-crystallin |
| UniProt Gene Name: | CRYBB1 |
| UniProt Entry Name: | CRBB1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CRYBB1 antibody (CAB2590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). |
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