Anti-CRY2 Antibody (CAB6891)
- SKU:
- CAB6891
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-CRY2 Antibody |
| 抗体コード: | CAB6891 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 455-614 of human CRY2 (NP_066940.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | HepG2, A-549, Rat heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 455-614 of human CRY2 (NP_066940.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | PVGF GRRT DPSG DYIR RYLP KLKA FPSR YIYE PWNA PESI QKAA KCII GVDY PRPI VNHA ETSR LNIE RMKQ IYQQ LSRY RGLC LLAS VPSC VEDL SHPV AEPS SSQA GSMS SAGP RPLP SGPA SPKR KLEA AEEP PGEE LSKR ARVA ELPT PELP SKDA |
| 遺伝子ID: | 1408 |
| Uniprot: | Q49AN0 |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 60kDa/66kDa |
| 観察された分子量: | 67KDa |
| 同義語: | CRY2, HCRY2, PHLL2 |
| バックグラウンド: | This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | CRY2: Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL. Belongs to the DNA photolyase class-1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; Lyase Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: cytoplasm; extracellular region; nucleus Molecular Function:ubiquitin binding; protein binding; DNA binding; DNA (6-4) photolyase activity; damaged DNA binding; deoxyribodipyrimidine photo-lyase activity; blue light photoreceptor activity; single-stranded DNA binding; phosphatase binding Biological Process: circadian rhythm; photoreactive repair; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; regulation of circadian rhythm; protein-chromophore linkage; entrainment of circadian clock by photoperiod; blue light signaling pathway; negative regulation of phosphoprotein phosphatase activity; negative regulation of circadian rhythm; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent |
| NCBI Summary: | This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014] |
| UniProt Code: | Q49AN0 |
| NCBI GenInfo Identifier: | 118572252 |
| NCBI Gene ID: | 1408 |
| NCBI Accession: | Q49AN0.2 |
| UniProt Secondary Accession: | Q49AN0,O75148, Q8IV71, B4DH32, B4DZD6, |
| UniProt Related Accession: | Q49AN0 |
| Molecular Weight: | |
| NCBI Full Name: | Cryptochrome-2 |
| NCBI Synonym Full Names: | cryptochrome circadian clock 2 |
| NCBI Official Symbol: | CRY2 |
| NCBI Official Synonym Symbols: | HCRY2; PHLL2 |
| NCBI Protein Information: | cryptochrome-2; growth-inhibiting protein 37; cryptochrome 2 (photolyase-like) |
| UniProt Protein Name: | Cryptochrome-2 |
| Protein Family: | Cryptochrome |
| UniProt Gene Name: | CRY2 |
| UniProt Entry Name: | CRY2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CRY2 antibody (CAB6891) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
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