Anti-CRKL Antibody (CAB0511)
- SKU:
- CAB0511
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-CRKL Antibody |
| 抗体コード: | CAB0511 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide of human CRKL |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | MCF7, HeLa, Raji, Mouse brain, Mouse thymus, Mouse liver |
| 免疫原: | A synthetic peptide of human CRKL |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 1399 |
| Uniprot: | P46109 |
| セルラーロケーション: | |
| 計算された分子量: | 33kDa |
| 観察された分子量: | 34kDa |
| 同義語: | CRKL |
| バックグラウンド: | This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic. |
| UniProt Protein Function: | CRKL: an oncogenic adaptor protein with an SH2-SH3-SH3 domain structure. Plays a specific role in integrin-induced migration as a downstream mediator of Src. Activates the RAS and Jnk signaling pathways. Transforms fibroblasts in a RAS-dependent fashion. A substrate of BCR-ABL and plays a role in fibroblast transformation by BCR-ABL. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: cytosol; endosome Molecular Function:protein binding; SH3/SH2 adaptor activity; signal transducer activity Biological Process: activation of MAPKK activity; JNK cascade; positive regulation of cell proliferation; Ras protein signal transduction |
| NCBI Summary: | This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009] |
| UniProt Code: | P46109 |
| NCBI GenInfo Identifier: | 1169094 |
| NCBI Gene ID: | 1399 |
| NCBI Accession: | P46109.1 |
| UniProt Secondary Accession: | P46109,A8KA44, D3DX35, |
| UniProt Related Accession: | P46109 |
| Molecular Weight: | 33,777 Da |
| NCBI Full Name: | Crk-like protein |
| NCBI Synonym Full Names: | CRK like proto-oncogene, adaptor protein |
| NCBI Official Symbol: | CRKL |
| NCBI Protein Information: | crk-like protein |
| UniProt Protein Name: | Crk-like protein |
| Protein Family: | Crk-like protein |
| UniProt Gene Name: | CRKL |
| UniProt Entry Name: | CRKL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CRKL antibody (CAB0511) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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