Anti-CPSF6 Antibody (CAB5963)
- SKU:
- CAB5963
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-CPSF6 Antibody |
| 抗体コード: | CAB5963 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF IP |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant protein of human CPSF6 |
| 申し込み: | WB IF IP |
| 推奨希釈: | WB 1:1000 - 1:3000 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, 293T, Jurkat, HepG2, HT-1080, Mouse brain, Mouse liver, Rat brain |
| 免疫原: | Recombinant protein of human CPSF6 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 11052 |
| Uniprot: | Q16630 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 52kDa/59kDa/63kDa |
| 観察された分子量: | 68kDa |
| 同義語: | CPSF6, CFIM, CFIM68, HPBRII-4, HPBRII-7, CFIM72 |
| バックグラウンド: | The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins. |
| UniProt Protein Function: | Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping. |
| NCBI Summary: | The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q16630 |
| NCBI GenInfo Identifier: | 665821177 |
| NCBI Gene ID: | 11052 |
| NCBI Accession: | NP_001287876.1 |
| UniProt Secondary Accession: | Q16630,Q53ES1, Q9BSJ7, Q9BW18, A8K7K9, |
| UniProt Related Accession: | Q16630 |
| Molecular Weight: | 52,326 Da |
| NCBI Full Name: | cleavage and polyadenylation specificity factor subunit 6 isoform 2 |
| NCBI Synonym Full Names: | cleavage and polyadenylation specific factor 6 |
| NCBI Official Symbol: | CPSF6 |
| NCBI Official Synonym Symbols: | CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7 |
| NCBI Protein Information: | cleavage and polyadenylation specificity factor subunit 6 |
| UniProt Protein Name: | Cleavage and polyadenylation specificity factor subunit 6 |
| UniProt Synonym Protein Names: | Cleavage and polyadenylation specificity factor 68 kDa subunit; CFIm68; CPSF 68 kDa subunit; Pre-mRNA cleavage factor Im 68 kDa subunit; Protein HPBRII-4/7 |
| Protein Family: | Cleavage and polyadenylation specificity factor |
| UniProt Gene Name: | CPSF6 |
 | Western blot analysis of extracts of various cell lines, using CPSF6 antibody (CAB5963) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Confocal immunofluorescence analysis of U2OS cells using CPSF6 Polyclonal Antibody (CAB5963) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug CPSF6 antibody (CAB5963). Western blot was performed from the immunoprecipitate using CPSF6 antibody (CAB5963) at a dilition of 1:1000. |
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