Anti-CPSF3 Antibody (CAB12368)
- SKU:
- CAB12368
- Product type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
抗体名: | Anti-CPSF3 Antibody |
抗体コード: | CAB12368 |
抗体サイズ: | 20uL, 50uL, 100uL |
申し込み: | WB |
反応性: | Mouse |
宿主種: | Rabbit |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 435-684 of human CPSF3 (NP_057291.1). |
申し込み: | WB |
推奨希釈: | WB 1:500 - 1:2000 |
反応性: | Mouse |
ポジティブサンプル: | Mouse liver, Mouse spleen |
免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 435-684 of human CPSF3 (NP_057291.1). |
精製方法: | Affinity purification |
ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
アイソタイプ: | IgG |
順序: | YEDN DEVH IEVH NPRN TEAV TLNF RGEK LAKV MGFL ADKK PEQG QRVS GILV KRNF NYHI LSPC DLSN YTDL AMST VKQT QAIP YTGP FNLL CYQL QKLT GDVE ELEI QEKP ALKV FKNI TVIQ EPGM VVLE WLAN PSND MYAD TVTT VILE VQSN PKIR KGAV QKVS KKLE MHVY SKRL EIML QDIF GEDC VSVK DDSI LSVT VDGK TANL NLET RTVE CEEG SEDD ESLR EMVE LAAQ RLYE ALTP VH |
遺伝子ID: | 51692 |
Uniprot: | Q9UKF6 |
セルラーロケーション: | Nucleus |
計算された分子量: | 77kDa |
観察された分子量: | 73kDa |
同義語: | CPSF3, CPSF-73, CPSF73 |
バックグラウンド: | This gene encodes a member of the metallo-beta-lactamase family. The encoded protein is a 73kDa subunit of the cleavage and polyadenylation specificity factor and functions as an endonuclease that recognizes the pre-mRNA 3'-cleavage site AAUAAA prior to polyadenylation. It also cleaves after the pre-mRNA sequence ACCCA during histone 3'-end pre-mRNA processing. |
UniProt Protein Function: | CPSF3: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP- dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily. |
UniProt Protein Details: | Protein type:EC 3.1.27.-; RNA processing; RNA splicing; RNA-binding; Ribonuclease Chromosomal Location of Human Ortholog: 2p25.1 Cellular Component: mRNA cleavage and polyadenylation specificity factor complex; nucleoplasm Molecular Function:5'-3' exonuclease activity; endoribonuclease activity; protein binding; RNA binding Biological Process: histone mRNA 3'-end processing; mRNA 3'-end processing; mRNA cleavage; mRNA export from nucleus; mRNA polyadenylation; nuclear mRNA splicing, via spliceosome; termination of RNA polymerase II transcription |
NCBI Summary: | This gene encodes a member of the metallo-beta-lactamase family. The encoded protein is a 73kDa subunit of the cleavage and polyadenylation specificity factor and functions as an endonuclease that recognizes the pre-mRNA 3'-cleavage site AAUAAA prior to polyadenylation. It also cleaves after the pre-mRNA sequence ACCCA during histone 3'-end pre-mRNA processing. [provided by RefSeq, Oct 2012] |
UniProt Code: | Q9UKF6 |
NCBI GenInfo Identifier: | 18203503 |
NCBI Gene ID: | 51692 |
NCBI Accession: | Q9UKF6.1 |
UniProt Secondary Accession: | Q9UKF6,O14769, Q53RS2, Q96F36, |
UniProt Related Accession: | Q9UKF6 |
Molecular Weight: | 77kDa |
NCBI Full Name: | Cleavage and polyadenylation specificity factor subunit 3 |
NCBI Synonym Full Names: | cleavage and polyadenylation specific factor 3 |
NCBI Official Symbol: | CPSF3 |
NCBI Official Synonym Symbols: | CPSF73; CPSF-73 |
NCBI Protein Information: | cleavage and polyadenylation specificity factor subunit 3 |
UniProt Protein Name: | Cleavage and polyadenylation specificity factor subunit 3 |
UniProt Synonym Protein Names: | Cleavage and polyadenylation specificity factor 73 kDa subunit; CPSF 73 kDa subunit; mRNA 3'-end-processing endonuclease CPSF-73 |
Protein Family: | Cleavage and polyadenylation specificity factor |
UniProt Gene Name: | CPSF3 |
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