Anti-COPE Antibody (CAB10047)
- SKU:
- CAB10047
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-COPE Antibody |
| 抗体コード: | CAB10047 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-308 of human COPE (NP_009194.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | SH-SY5Y, 293T, HeLa, Raji, LO2, Mouse pancreas |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-308 of human COPE (NP_009194.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAPP APGP ASGG SGEV DELF DVKN AFYI GSYQ QCIN EAQR VKLS SPER DVER DVFL YRAY LAQR KFGV VLDE IKPS SAPE LQAV RMFA DYLA HESR RDSI VAEL DREM SRSV DVTN TTFL LMAA SIYL HDQN PDAA LRAL HQGD SLEC TAMT VQIL LKLD RLDL ARKE LKRM QDLD EDAT LTQL ATAW VSLA TGGE KLQD AYYI FQEM ADKC SPTL LLLN GQAA CHMA QGRW EAAE GLLQ EALD KDSG YPET LVNL IVLS QHLG KPPE VTNR YLSQ LKDA HRSH PFIK EYQA KEND FDRL VLQY APSA |
| 遺伝子ID: | 11316 |
| Uniprot: | O14579 |
| セルラーロケーション: | COPI-coated vesicle membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus membrane, Peripheral membrane protein |
| 計算された分子量: | 28kDa/34kDa |
| 観察された分子量: | 34kDa |
| 同義語: | COPE, epsilon-COP |
| バックグラウンド: | The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| UniProt Protein Function: | COPE: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Belongs to the COPE family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 19p13.11 Cellular Component: COPI vesicle coat; cytosol; endoplasmic reticulum membrane; Golgi apparatus; Golgi membrane; nucleoplasm; transport vesicle Molecular Function:protein binding Biological Process: ER to Golgi vesicle-mediated transport; intra-Golgi vesicle-mediated transport; retrograde vesicle-mediated transport, Golgi to ER |
| NCBI Summary: | The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O14579 |
| NCBI GenInfo Identifier: | 6647482 |
| NCBI Gene ID: | 11316 |
| NCBI Accession: | O14579.3 |
| UniProt Secondary Accession: | O14579,O76097, Q6IBB8, Q9UGP6, A6NE29, A6NKA3, |
| UniProt Related Accession: | O14579 |
| Molecular Weight: | 28,773 Da |
| NCBI Full Name: | Coatomer subunit epsilon |
| NCBI Synonym Full Names: | coatomer protein complex subunit epsilon |
| NCBI Official Symbol: | COPE |
| NCBI Official Synonym Symbols: | epsilon-COP |
| NCBI Protein Information: | coatomer subunit epsilon |
| UniProt Protein Name: | Coatomer subunit epsilon |
| UniProt Synonym Protein Names: | Epsilon-coat protein; Epsilon-COP |
| Protein Family: | Coatomer |
| UniProt Gene Name: | COPE |
| UniProt Entry Name: | COPE_HUMAN |
 | Western blot analysis of extracts of various cell lines, using COPE antibody (CAB10047) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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