Anti-COPB2 Antibody (CAB7036)
- SKU:
- CAB7036
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-COPB2 Antibody |
| 抗体コード: | CAB7036 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 657-906 of human COPB2 (NP_004757.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | SW480, MCF7, HeLa, HepG2, Jurkat, Mouse liver, Mouse kidney, Mouse stomach |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 657-906 of human COPB2 (NP_004757.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | AYQL AVEA ESEQ KWKQ LAEL AISK CQFG LAQE CLHH AQDY GGLL LLAT ASGN ANMV NKLA EGAE RDGK NNVA FMSY FLQG KVDA CLEL LIRT GRLP EAAF LART YLPS QVSR VVKL WREN LSKV NQKA AESL ADPT EYEN LFPG LKEA FVVE EWVK ETHA DLWP AKQY PLVT PNEE RNVM EEGK DFQP SRST AQQE LDGK PASP TPVI VASH TANK EEKS LLEL EVDL DNLE LEDI DTTD INLD EDIL DD |
| 遺伝子ID: | 9276 |
| Uniprot: | P35606 |
| セルラーロケーション: | COPI-coated vesicle membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus membrane, Peripheral membrane protein, cytosol |
| 計算された分子量: | 99kDa/102kDa |
| 観察された分子量: | 102kDa |
| 同義語: | COPB2, beta-COP |
| バックグラウンド: | The Golgi coatomer complex (see MIM 601924) constitutes the coat of nonclathrin-coated vesicles and is essential for Golgi budding and vesicular trafficking. It consists of 7 protein subunits, including COPB2.[supplied by OMIM, Jul 2002] |
| UniProt Protein Function: | COPB2: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Belongs to the WD repeat COPB2 family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Vesicle Chromosomal Location of Human Ortholog: 3q23 Cellular Component: COPI vesicle coat; cytosol; endoplasmic reticulum membrane; Golgi membrane; transport vesicle Molecular Function:protein binding Biological Process: ER to Golgi vesicle-mediated transport; intra-Golgi vesicle-mediated transport; intracellular protein transport; retrograde vesicle-mediated transport, Golgi to ER |
| NCBI Summary: | The Golgi coatomer complex (see MIM 601924) constitutes the coat of nonclathrin-coated vesicles and is essential for Golgi budding and vesicular trafficking. It consists of 7 protein subunits, including COPB2.[supplied by OMIM, Jul 2002] |
| UniProt Code: | P35606 |
| NCBI GenInfo Identifier: | 544076 |
| NCBI Gene ID: | 9276 |
| NCBI Accession: | P35606.2 |
| UniProt Secondary Accession: | P35606,B4DZI8, |
| UniProt Related Accession: | P35606 |
| Molecular Weight: | 99,046 Da |
| NCBI Full Name: | Coatomer subunit beta' |
| NCBI Synonym Full Names: | coatomer protein complex subunit beta 2 |
| NCBI Official Symbol: | COPB2 |
| NCBI Official Synonym Symbols: | beta'-COP |
| NCBI Protein Information: | coatomer subunit beta' |
| UniProt Protein Name: | Coatomer subunit beta' |
| UniProt Synonym Protein Names: | Beta'-coat protein; Beta'-COP; p102 |
| UniProt Gene Name: | COPB2 |
| UniProt Entry Name: | COPB2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using COPB2 antibody (CAB7036) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded rat brain using COPB2 antibody (CAB7036) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using COPB2 antibody (CAB7036) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using COPB2 antibody (CAB7036) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using COPB2 antibody (CAB7036) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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