Anti-COMT Antibody (RACO0511)
- SKU:
- RACO0511
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
| 商品名: | COMT Antibody |
| Product SKU: | RACO0511 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human COMT |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. |
| シノニム: | Catechol O-methyltransferase (EC 2.1.1.6), COMT |
 | IHC image of RACO0511 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Western Blot. Positive WB detected in 293 whole cell lysate THP-1 whole cell lysate HepG2 whole cell lysate All lanes COMT Antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 31, 25 kDa. Observed band size: 31 kDa. |
| UniProt Protein Function: | COMT: Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. Belongs to the mammalian catechol-O-methyltransferase family. 2 isoforms of the human protein are produced by alternative initiation. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Methyltransferase; EC 2.1.1.6; Amino Acid Metabolism - tyrosine Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: postsynaptic membrane; mitochondrion; membrane; axon; plasma membrane; dendritic spine; integral to membrane; cytosol Molecular Function:protein binding; magnesium ion binding; O-methyltransferase activity; catechol O-methyltransferase activity Biological Process: response to drug; methylation; estrogen metabolic process; cellular response to phosphate starvation; neurotransmitter catabolic process; dopamine catabolic process; negative regulation of smooth muscle cell proliferation; short-term memory; response to pain; response to lipopolysaccharide; female pregnancy; learning; reproductive process in a multicellular organism; response to organic cyclic substance; positive regulation of homocysteine metabolic process; negative regulation of dopamine metabolic process; synaptic transmission; xenobiotic metabolic process; regulation of sensory perception of pain; developmental process; neurotransmitter biosynthetic process Disease: Schizophrenia; Panic Disorder 1 |
| NCBI Summary: | Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008] |
| UniProt Code: | P21964 |
| NCBI GenInfo Identifier: | 116907 |
| NCBI Gene ID: | 1312 |
| NCBI Accession: | P21964.2 |
| UniProt Secondary Accession: | P21964,Q6IB07, Q6ICE6, Q9BWC7, A8MPV9, |
| UniProt Related Accession: | P21964 |
| Molecular Weight: | 271 |
| NCBI Full Name: | Catechol O-methyltransferase |
| NCBI Synonym Full Names: | catechol-O-methyltransferase |
| NCBI Official Symbol: | COMT |
| NCBI Official Synonym Symbols: | HEL-S-98n |
| NCBI Protein Information: | catechol O-methyltransferase; epididymis secretory sperm binding protein Li 98n |
| UniProt Protein Name: | Catechol O-methyltransferase |
| Protein Family: | Catechol O-methyltransferase |
| UniProt Gene Name: | COMT |
| UniProt Entry Name: | COMT_HUMAN |