Anti-CLOCK Antibody (CAB7265)

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SKU:
CAB7265
Product type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
KAT13D
Synonyms:
bHLHe8
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Description

I抗体名:CLOCK Rabbit mAb
抗体コード:CAB7265
サイズ:20uL, 50uL, 100uL
同義語:KAT13D, bHLHe8
申し込み:WB, IF
反応性:Human, Mouse, Rat
宿主種:Rabbit
免疫原:A synthesized peptide derived from human CLOCK
申し込み:WB, IF
推奨される希釈:WB 1:500 - 1:2000 IF 1:50 - 1:200
反応性:Human, Mouse, Rat
ポジティブサンプル:HeLa, 293T, NIH/3T3, SH-SY5Y, Mouse lung, Rat brain
免疫原:A synthesized peptide derived from human CLOCK
精製方法:Affinity purification
保管所:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:Email for sequence
遺伝子ID:9575
Uniprot:O15516
セルラーロケーション:Cytoplasm, Nucleus
計算された分子量:95kDa
観察された分子量:95kDa
UniProt Protein Function:CLOCK: ARNTL/2-CLOCK heterodimers activate E-box element (3'- CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression. Acetylates primarily histones H3 and H4. Acetylates also a non-histone substrate: ARNTL. Plays a role in DNA damage response (DDR) signaling during the S phase. Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with ARNTL is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and ARNTL. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-ARNTL heterodimer with PER or CRY inhibits transcription activation. Binds weakly ARNTL and ARNTL2 to form heterodimers which bind poorly to the E-box motif. Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).
UniProt Protein Details:

Protein type:EC 2.3.1.48; DNA-binding; Acetyltransferase; Transcription factor

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: chromosome; intracellular membrane-bound organelle; nucleoplasm; nucleus; transcription factor complex

Molecular Function:chromatin DNA binding; DNA binding; histone acetyltransferase activity; protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: activation of NF-kappaB transcription factor; circadian regulation of gene expression; circadian rhythm; DNA damage checkpoint; negative regulation of transcription, DNA-dependent; photoperiodism; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; regulation of hair cycle; regulation of insulin secretion; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to redox state; signal transduction; spermatogenesis

NCBI Summary:The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Code:O15516
NCBI GenInfo Identifier:6166009
NCBI Gene ID:9575
NCBI Accession:O15516.1
UniProt Secondary Accession:O15516,O14516, Q9UIT8, A0AV01, A2I2N9,
UniProt Related Accession:O15516
Molecular Weight:95,304 Da
NCBI Full Name:Circadian locomoter output cycles protein kaput
NCBI Synonym Full Names:clock circadian regulator
NCBI Official Symbol:CLOCK  
NCBI Official Synonym Symbols:KAT13D; bHLHe8  
NCBI Protein Information:circadian locomoter output cycles protein kaput
UniProt Protein Name:Circadian locomoter output cycles protein kaput
UniProt Synonym Protein Names:Class E basic helix-loop-helix protein 8; bHLHe8
Protein Family:Clock-controlled protein
UniProt Gene Name:CLOCK  
UniProt Entry Name:CLOCK_HUMAN
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