Anti-CLOCK Antibody (CAB5633)
- SKU:
- CAB5633
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-CLOCK Antibody |
| 抗体コード: | CAB5633 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 577-846 of human CLOCK (NP_004889.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HepG2, A-549, SH-SY5Y, Mouse liver, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 577-846 of human CLOCK (NP_004889.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | GSVQ LSSG NSSN IQQL APIN MQGQ VVPT NQIQ SGMN TGHI GTTQ HMIQ QQTL QSTS TQSQ QNVL SGHS QQTS LPSQ TQST LTAP LYNT MVIS QPAA GSMV QIPS SMPQ NSTQ SAAV TTFT QDRQ IRFS QGQQ LVTK LVTA PVAC GAVM VPST MLMG QVVT AYPT FATQ QQQS QTLS VTQQ QQQQ SSQE QQLT SVQQ PSQA QLTQ PPQQ FLQT SRLL HGNP STQL ILSA AFPL QQST FPQS HHQQ HQSQ QQQQ LSRH RTDS LPDP SKVQ PQ |
| 遺伝子ID: | 9575 |
| Uniprot: | O15516 |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 95kDa |
| 観察された分子量: | 110kDa |
| 同義語: | CLOCK, KAT13D, bHLHe8 |
| バックグラウンド: | The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | CLOCK: ARNTL/2-CLOCK heterodimers activate E-box element (3'- CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression. Acetylates primarily histones H3 and H4. Acetylates also a non-histone substrate: ARNTL. Plays a role in DNA damage response (DDR) signaling during the S phase. Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with ARNTL is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and ARNTL. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-ARNTL heterodimer with PER or CRY inhibits transcription activation. Binds weakly ARNTL and ARNTL2 to form heterodimers which bind poorly to the E-box motif. Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN). |
| UniProt Protein Details: | Protein type:EC 2.3.1.48; DNA-binding; Acetyltransferase; Transcription factor Chromosomal Location of Human Ortholog: 4q12 Cellular Component: chromosome; intracellular membrane-bound organelle; nucleoplasm; nucleus; transcription factor complex Molecular Function:chromatin DNA binding; DNA binding; histone acetyltransferase activity; protein binding; sequence-specific DNA binding; transcription factor activity Biological Process: activation of NF-kappaB transcription factor; circadian regulation of gene expression; circadian rhythm; DNA damage checkpoint; negative regulation of transcription, DNA-dependent; photoperiodism; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; regulation of hair cycle; regulation of insulin secretion; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to redox state; signal transduction; spermatogenesis |
| NCBI Summary: | The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| UniProt Code: | O15516 |
| NCBI GenInfo Identifier: | 6166009 |
| NCBI Gene ID: | 9575 |
| NCBI Accession: | O15516.1 |
| UniProt Secondary Accession: | O15516,O14516, Q9UIT8, A0AV01, A2I2N9, |
| UniProt Related Accession: | O15516 |
| Molecular Weight: | 95,304 Da |
| NCBI Full Name: | Circadian locomoter output cycles protein kaput |
| NCBI Synonym Full Names: | clock circadian regulator |
| NCBI Official Symbol: | CLOCK |
| NCBI Official Synonym Symbols: | KAT13D; bHLHe8 |
| NCBI Protein Information: | circadian locomoter output cycles protein kaput |
| UniProt Protein Name: | Circadian locomoter output cycles protein kaput |
| UniProt Synonym Protein Names: | Class E basic helix-loop-helix protein 8; bHLHe8 |
| Protein Family: | Clock-controlled protein |
| UniProt Gene Name: | CLOCK |
| UniProt Entry Name: | CLOCK_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CLOCK antibody (CAB5633) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of extracts of Rat liver, using CLOCK antibody (CAB5633) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
View AllClose