Anti-Claudin-18 Antibody (CAB8392)
- SKU:
- CAB8392
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-Claudin-18 Antibody |
| 抗体コード: | CAB8392 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-80 of human CLDN18 (NP_057453.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:1000 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HL-60, 293T, B cells, Mouse liver, Mouse kidney, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-80 of human CLDN18 (NP_057453.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | GCIA ATGM DMWS TQDL YDNP VTSV FQYE GLWR SCVR QSSG FTEC RPYF TILG LPAM LQAV R |
| 遺伝子ID: | 51208 |
| Uniprot: | P56856 |
| セルラーロケーション: | Cell junction, Cell membrane, Multi-pass membrane protein, tight junction |
| 計算された分子量: | 27kDa |
| 観察された分子量: | 28kDa |
| 同義語: | CLDN18, SFTA5, SFTPJ |
| バックグラウンド: | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| UniProt Protein Function: | Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. |
| NCBI Summary: | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2010] |
| UniProt Code: | P56856 |
| NCBI GenInfo Identifier: | 7387578 |
| NCBI Gene ID: | 51208 |
| NCBI Accession: | P56856.1 |
| UniProt Secondary Accession: | P56856,Q96PH4, A5PL21, |
| UniProt Related Accession: | P56856 |
| Molecular Weight: | 27,720 Da |
| NCBI Full Name: | Claudin-18 |
| NCBI Synonym Full Names: | claudin 18 |
| NCBI Official Symbol: | CLDN18 |
| NCBI Official Synonym Symbols: | SFTA5; SFTPJ |
| NCBI Protein Information: | claudin-18 |
| UniProt Protein Name: | Claudin-18 |
| Protein Family: | Claudin |
| UniProt Gene Name: | CLDN18 |
| UniProt Entry Name: | CLD18_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CLDN18 antibody (CAB8392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |