Anti-CLASP1 Antibody (CAB7081)
- SKU:
- CAB7081
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-CLASP1 Antibody |
| 抗体コード: | CAB7081 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1389-1538 of human CLASP1 (NP_056097.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HepG2, MCF7, Mouse brain, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1389-1538 of human CLASP1 (NP_056097.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | IMKT LEAH KDSH KEVV RAAE EAAS TLAS SIHP EQCI KVLC PIIQ TADY PINL AAIK MQTK VVER IAKE SLLQ LLVD IIPG LLQG YDNT ESSV RKAS VFCL VAIY SVIG EDLK PHLA QLTG SKMK LLNL YIKR AQTT NSNS SSSS DVST HS |
| 遺伝子ID: | 23332 |
| Uniprot: | Q7Z460 |
| セルラーロケーション: | Chromosome, Cytoplasm, Golgi apparatus, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, spindle, trans-Golgi network |
| 計算された分子量: | 162kDa/163kDa/164kDa/169kDa |
| 観察された分子量: | 169kDa |
| 同義語: | CLASP1, MAST1 |
| バックグラウンド: |
| UniProt Protein Function: | CLASP1: Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Belongs to the CLASP family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2q14.2-q14.3 Cellular Component: kinetochore; kinetochore microtubule; Golgi apparatus; centrosome; membrane; cytoplasmic microtubule; spindle microtubule; cortical microtubule cytoskeleton; cell cortex; cytosol Molecular Function:kinetochore binding; microtubule plus-end binding; protein binding; microtubule binding Biological Process: axon guidance; microtubule organizing center organization and biogenesis; negative regulation of microtubule depolymerization; organelle organization and biogenesis; negative regulation of microtubule polymerization or depolymerization; establishment and/or maintenance of cell polarity; establishment of spindle orientation; microtubule cytoskeleton organization and biogenesis; microtubule nucleation; cell division; mitotic cell cycle; G2/M transition of mitotic cell cycle; exit from mitosis; microtubule bundle formation |
| NCBI Summary: | CLASPs, such as CLASP1, are nonmotor microtubule-associated proteins that interact with CLIPs (e.g., CLIP170; MIM 179838). CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle (Maiato et al., 2003 [PubMed 12837247]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q7Z460 |
| NCBI GenInfo Identifier: | 74723323 |
| NCBI Gene ID: | 23332 |
| NCBI Accession: | Q7Z460.1 |
| UniProt Secondary Accession: | Q7Z460,O75118, Q2KHQ9, Q5H9P0, Q8N5B8, Q9BQT5, A2RU21 B7ZLX3, F5GWS0, |
| UniProt Related Accession: | Q7Z460 |
| Molecular Weight: | 1538 |
| NCBI Full Name: | CLIP-associating protein 1 |
| NCBI Synonym Full Names: | cytoplasmic linker associated protein 1 |
| NCBI Official Symbol: | CLASP1 |
| NCBI Official Synonym Symbols: | MAST1 |
| NCBI Protein Information: | CLIP-associating protein 1; multiple asters 1; protein Orbit homolog 1; multiple asters homolog 1; cytoplasmic linker-associated protein 1 |
| UniProt Protein Name: | CLIP-associating protein 1 |
| UniProt Synonym Protein Names: | Cytoplasmic linker-associated protein 1; Multiple asters homolog 1; Protein Orbit homolog 1; hOrbit1 |
| Protein Family: | CLIP-associating protein |
| UniProt Gene Name: | CLASP1 |
| UniProt Entry Name: | CLAP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CLASP1 antibody (CAB7081) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded mouse ileum using CLASP1 antibody (CAB7081) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using CLASP1 antibody (CAB7081). Blue: DAPI for nuclear staining. |
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