Anti-CKMT1B 抗体 (CAB3046)

抗体名:	Anti-CKMT1B Antibody
抗体コード:	CAB3046
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IF
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human CKMT1B (NP_066270.1).

申し込み:	WB IHC IF
推奨希釈:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
反応性:	Human, Mouse, Rat
ポジティブサンプル:	Mouse brain, Mouse kidney, Rat brain

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human CKMT1B (NP_066270.1).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	MAGP FSRL LSAR PGLR LLAL AGAG SLAA GFLL RPEP VRAA SERR RLYP PSAE YPDL RKHN NCMA SHLT PAVY ARLC DKTT PTGW T
遺伝子ID:	1159
Uniprot:	P12532
セルラーロケーション:	Intermembrane side, Mitochondrion inner membrane, Peripheral membrane protein
計算された分子量:	47kDa/50kDa
観察された分子量:	40kDa

同義語:

CKMT1B, CKMT, CKMT1, UMTCK

バックグラウンド:

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins.

UniProt Protein Function:	Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
NCBI Summary:	Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq, Jul 2008]
UniProt Code:	P12532
NCBI GenInfo Identifier:	125315
NCBI Gene ID:	548596
NCBI Accession:	P12532.1
UniProt Secondary Accession:	P12532,Q0VAM3, Q32NF6, Q53FC4, B4DIT8, B7ZA09,
UniProt Related Accession:	P12532
Molecular Weight:	50,421 Da
NCBI Full Name:	Creatine kinase U-type, mitochondrial
NCBI Synonym Full Names:	creatine kinase, mitochondrial 1A
NCBI Official Symbol:	CKMT1A
NCBI Official Synonym Symbols:	CKMT1; U-MtCK; mia-CK
NCBI Protein Information:	creatine kinase U-type, mitochondrial
UniProt Protein Name:	Creatine kinase U-type, mitochondrial
UniProt Synonym Protein Names:	Acidic-type mitochondrial creatine kinase; Mia-CK; Ubiquitous mitochondrial creatine kinase; U-MtCK
UniProt Gene Name:	CKMT1A
UniProt Entry Name:	KCRU_HUMAN

	Western blot analysis of extracts of various cell lines, using CKMT1B antibody (CAB3046) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded human colon using CKMT1B antibody (CAB3046) at dilution of 1:150 (40x lens).
	Immunofluorescence analysis of NIH-3T3 cells using CKMT1B Polyclonal Antibody (CAB3046) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using CKMT1B Polyclonal Antibody (CAB3046) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-CKMT1A Antibody (CAB5233)

CKMT1A Antibody (PACO08447)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)