Anti-CHST15 Antibody (CAB8984)
- SKU:
- CAB8984
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-CHST15 Antibody |
| 抗体コード: | CAB8984 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 452-561 of human CHST15 (NP_056976.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | Raji, Rat heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 452-561 of human CHST15 (NP_056976.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | LQVG LYAV YLLD WLSV FDKQ QFLI LRLE DHAS NVKY TMHK VFQF LNLG PLSE KQEA LMTK SPAS NARR PEDR NLGP MWPI TQKI LRDF YRPF NARL AQVL ADEA FAWK TT |
| 遺伝子ID: | 51363 |
| Uniprot: | Q7LFX5 |
| セルラーロケーション: | Golgi apparatus membrane, Single-pass type II membrane protein |
| 計算された分子量: | 58kDa/64kDa |
| 観察された分子量: | 80kDa |
| 同義語: | CHST15, BRAG, GALNAC4S-6ST |
| バックグラウンド: | Chondroitin sulfate (CS) is a glycosaminoglycan which is an important structural component of the extracellular matrix and which links to proteins to form proteoglycans. Chondroitin sulfate E (CS-E) is an isomer of chondroitin sulfate in which the C-4 and C-6 hydroxyl groups are sulfated. This gene encodes a type II transmembrane glycoprotein that acts as a sulfotransferase to transfer sulfate to the C-6 hydroxal group of chondroitin sulfate. This gene has also been identified as being co-expressed with RAG1 in B-cells and as potentially acting as a B-cell surface signaling receptor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | CHST15: Sulfotransferase that transfers sulfate from 3'- phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non- reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B- cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo. Belongs to the sulfotransferase 1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.8.2.33; Transferase; Glycan Metabolism - chondroitin sulfate biosynthesis; Membrane protein, integral Chromosomal Location of Human Ortholog: 10q26 Cellular Component: Golgi membrane; integral to membrane Molecular Function:N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity; 3'-phosphoadenosine 5'-phosphosulfate binding Biological Process: chondroitin sulfate metabolic process; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; hexose biosynthetic process; pathogenesis |
| NCBI Summary: | Chondroitin sulfate (CS) is a glycosaminoglycan which is an important structural component of the extracellular matrix and which links to proteins to form proteoglycans. Chondroitin sulfate E (CS-E) is an isomer of chondroitin sulfate in which the C-4 and C-6 hydroxyl groups are sulfated. This gene encodes a type II transmembrane glycoprotein that acts as a sulfotransferase to transfer sulfate to the C-6 hydroxal group of chondroitin sulfate. This gene has also been identified as being co-expressed with RAG1 in B-cells and as potentially acting as a B-cell surface signaling receptor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012] |
| UniProt Code: | Q7LFX5 |
| NCBI GenInfo Identifier: | 74749920 |
| NCBI Gene ID: | 51363 |
| NCBI Accession: | Q7LFX5.1 |
| UniProt Secondary Accession: | Q7LFX5,O60338, O60474, Q86VM4, |
| UniProt Related Accession: | Q7LFX5 |
| Molecular Weight: | 58,109 Da |
| NCBI Full Name: | Carbohydrate sulfotransferase 15 |
| NCBI Synonym Full Names: | carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 |
| NCBI Official Symbol: | CHST15 |
| NCBI Official Synonym Symbols: | BRAG; GALNAC4S-6ST |
| NCBI Protein Information: | carbohydrate sulfotransferase 15; B cell RAG associated protein (GALNAC4S-6ST); B-cell RAG-associated gene protein; hBRAG |
| UniProt Protein Name: | Carbohydrate sulfotransferase 15 |
| UniProt Synonym Protein Names: | B-cell RAG-associated gene protein; hBRAG; N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase; GalNAc4S-6ST |
| Protein Family: | Carbohydrate sulfotransferase |
| UniProt Gene Name: | CHST15 |
| UniProt Entry Name: | CHSTF_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CHST15 antibody (CAB8984) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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