Anti-CHRNB2 Antibody (CAB9808)
- SKU:
- CAB9808
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Description
| 抗体名: | Anti-CHRNB2 Antibody |
| 抗体コード: | CAB9808 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 340-430 of human CHRNB2 (NP_000739.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HepG2, SH-SY5Y, Mouse liver, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 340-430 of human CHRNB2 (NP_000739.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | LEKL PALL FMQQ PRHH CARQ RLRL RRRQ RERE GAGA LFFR EAPG ADSC TCFV NRAS VQGL AGAF GAEP APVA GPGR SGEP CGCG LREA VDG |
| 遺伝子ID: | 1141 |
| Uniprot: | P17787 |
| セルラーロケーション: | Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse |
| 計算された分子量: | 57kDa |
| 観察された分子量: | 50-52kDa |
| 同義語: | CHRNB2, EFNL3, nAChRB2 |
| バックグラウンド: | Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy. |
| UniProt Protein Function: | nAChRB2: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRNB2 are the cause of nocturnal frontal lobe epilepsy type 3 (ENFL3). ENFL3 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta- 2/CHRNB2 sub-subfamily. |
| UniProt Protein Details: | Protein type:Receptor, misc.; Channel, cation; Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral Chromosomal Location of Human Ortholog: 1q21.3 Cellular Component: external side of plasma membrane; nicotinic acetylcholine-gated receptor-channel complex; plasma membrane Molecular Function:acetylcholine binding; acetylcholine receptor activity; ligand-gated ion channel activity; nicotinic acetylcholine-activated cation-selective channel activity; protein binding Biological Process: associative learning; B cell activation; behavioral response to nicotine; calcium ion transport; central nervous system projection neuron axonogenesis; cognition; lateral geniculate nucleus development; learning; locomotory behavior; membrane depolarization; memory; negative regulation of action potential; neurological system process; neuromuscular synaptic transmission; optic nerve morphogenesis; positive regulation of B cell proliferation; positive regulation of dopamine secretion; regulation of circadian sleep/wake cycle, REM sleep; regulation of dendrite morphogenesis; regulation of dopamine metabolic process; regulation of dopamine secretion; regulation of synaptic transmission, dopaminergic; regulation of synaptogenesis; response to cocaine; response to ethanol; response to hypoxia; response to nicotine; sensory perception of pain; sensory perception of sound; signal transduction; smooth muscle contraction; social behavior; synaptic transmission involved in micturition; synaptic transmission, cholinergic; vestibulocochlear nerve development; visual learning; visual perception Disease: Epilepsy, Nocturnal Frontal Lobe, 3 |
| NCBI Summary: | Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P17787 |
| NCBI GenInfo Identifier: | 113105 |
| NCBI Gene ID: | 1141 |
| NCBI Accession: | P17787.1 |
| UniProt Secondary Accession: | P17787,Q9UEH9, |
| UniProt Related Accession: | P17787 |
| Molecular Weight: | 57,019 Da |
| NCBI Full Name: | Neuronal acetylcholine receptor subunit beta-2 |
| NCBI Synonym Full Names: | cholinergic receptor nicotinic beta 2 subunit |
| NCBI Official Symbol: | CHRNB2 |
| NCBI Official Synonym Symbols: | EFNL3; nAChRB2 |
| NCBI Protein Information: | neuronal acetylcholine receptor subunit beta-2 |
| UniProt Protein Name: | Neuronal acetylcholine receptor subunit beta-2 |
| Protein Family: | Neuronal acetylcholine receptor |
| UniProt Gene Name: | CHRNB2 |
| UniProt Entry Name: | ACHB2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CHRNB2 antibody (CAB9808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using CHRNB2 antibody (CAB9808) at dilution of 1:100 (40x lens). |