Anti-CHRNA7 Antibody (CAB1588)
- SKU:
- CAB1588
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-CHRNA7 Antibody |
| 抗体コード: | CAB1588 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-230 of human CHRNA7 (NP_001177384.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse brain, Mouse heart, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-230 of human CHRNA7 (NP_001177384.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | ASPP STPP WDPG HIPG ASVR PAPG PVSL QGEF QRKL YKEL VKNY NPLE RPVA NDSQ PLTV YFSL SLLQ IMDV DEKN QVLT TNIW LQMS WTDH YLQW NVSE YPGV KTVR FPDG QIWK PDIL LYNS ADER FDAT FHTN VLVN SSGH CQYL PPGI FKSS CYID VRWF PFDV QHCK LKFG SWSY GGWS LDLQ MQEA DISG YIPN GEWD LVGI |
| 遺伝子ID: | 1139 |
| Uniprot: | P36544 |
| セルラーロケーション: | Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse |
| 計算された分子量: | 11kDa/56kDa/59kDa |
| 観察された分子量: | 53kDa |
| 同義語: | CHRNA7, CHRNA7-2, NACHRA7 |
| バックグラウンド: | The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | nAChRA7: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 7/CHRNA7 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Channel, cation; Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral Chromosomal Location of Human Ortholog: 15q14 Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; plasma membrane; integral to membrane; cell junction Molecular Function:toxin binding; chloride channel regulator activity; protein homodimerization activity; beta-amyloid binding; acetylcholine-gated cation channel activity; acetylcholine receptor activity; nicotinic acetylcholine-activated cation-selective channel activity; acetylcholine binding Biological Process: response to nicotine; cellular calcium ion homeostasis; synaptic transmission; positive regulation of angiogenesis; activation of MAPK activity; calcium ion transport; positive regulation of cell proliferation; response to hypoxia; ion transport; negative regulation of tumor necrosis factor production; signal transduction; cognition; memory Disease: Chromosome 15q13.3 Deletion Syndrome |
| NCBI Summary: | The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The family member CHRNA7, which is located on chromosome 15 in a region associated with several neuropsychiatric disorders, is partially duplicated and forms a hybrid with a novel gene from the family with sequence similarity 7 (FAM7A). Alternative splicing has been observed, and two variants exist, for this hybrid gene. The N-terminally truncated products predicted by the largest open reading frames for each variant would lack the majority of the neurotransmitter-gated ion-channel ligand binding domain but retain the transmembrane region that forms the ion channel. Although current evidence supports transcription of this hybrid gene, translation of the nicotinic acetylcholine receptor-like protein-encoding open reading frames has not been confirmed. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P36544 |
| NCBI GenInfo Identifier: | 2506127 |
| NCBI Gene ID: | 89832 |
| NCBI Accession: | P36544.5 |
| UniProt Secondary Accession: | P36544,Q15826, Q8IUZ4, Q96RH2, Q99555, Q9BXH0, A8K7Q4 B4DFS0, |
| UniProt Related Accession: | Q494W8 |
| Molecular Weight: | 502 |
| NCBI Full Name: | Neuronal acetylcholine receptor subunit alpha-7 |
| NCBI Synonym Full Names: | CHRNA7 (cholinergic receptor, nicotinic, alpha 7, exons 5-10) and FAM7A (family with sequence similarity 7A, exons A-E) fusion |
| NCBI Official Symbol: | CHRFAM7A |
| NCBI Official Synonym Symbols: | D-10; CHRNA7; CHRNA7-DR1 |
| NCBI Protein Information: | CHRNA7-FAM7A fusion protein; alpha-7 nicotinic cholinergic receptor subunit; alpha 7 neuronal nicotinic acetylcholine receptor-FAM7A hybrid; CHRNA7 (cholinergic receptor, nicotinic, alpha polypeptide 7, exons 5-10) and FAM7A (family with sequence similari |
| UniProt Protein Name: | Neuronal acetylcholine receptor subunit alpha-7 |
| Protein Family: | CHRNA7-FAM7A fusion protein |
| UniProt Gene Name: | CHRNA7 |
| UniProt Entry Name: | ACHA7_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CHRNA7 antibody (CAB1588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded human placenta using CHRNA7 Rabbit pAb (CAB1588) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of L929 cells using CHRNA7 antibody (CAB1588) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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