Anti-CHCHD4 Antibody (CAB13139)
- SKU:
- CAB13139
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-CHCHD4 Antibody |
| 抗体コード: | CAB13139 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human CHCHD4 (NP_001091972.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | LO2, 293T, Mouse liver, Mouse heart, Mouse pancreas |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human CHCHD4 (NP_001091972.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MSYC RQEG KDRI IFVT KEDH ETPS SAEL VADD PNDP YEEH GLIL PNGN INWN CPCL GGMA SGPC GEQF KSAF SCFH YSTE EIKG SDCV DQFR AMQE CMQK YPDL YPQE DEDE EEER EKKP AEQA EETA PIEA TATK EEEG SS |
| 遺伝子ID: | 131474 |
| Uniprot: | Q8N4Q1 |
| セルラーロケーション: | Mitochondrion intermembrane space |
| 計算された分子量: | 15kDa/17kDa |
| 観察された分子量: | 16kDa |
| 同義語: | CHCHD4, MIA40, TIMM40 |
| バックグラウンド: |
| UniProt Protein Function: | CHCHD4: Required for the import and folding of small cysteine- containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Probably acts by forming a redox cycle with GFER/ERV1 that involves a disulfide relay system. Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS (Probable). 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial Chromosomal Location of Human Ortholog: 3p25.1 Cellular Component: mitochondrial intermembrane space; mitochondrion Molecular Function:protein disulfide oxidoreductase activity Biological Process: 'de novo' posttranslational protein folding; protein import into mitochondrial intermembrane space; protein maturation via protein folding |
| NCBI Summary: | CHCHD4, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus (Hofmann et al., 2005 [PubMed 16185709]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q8N4Q1 |
| NCBI GenInfo Identifier: | 62510498 |
| NCBI Gene ID: | 131474 |
| NCBI Accession: | Q8N4Q1.1 |
| UniProt Secondary Accession: | Q8N4Q1,Q96AI2, Q96MY6, A8K3Z9, |
| UniProt Related Accession: | Q8N4Q1 |
| Molecular Weight: | 17,385 Da |
| NCBI Full Name: | Mitochondrial intermembrane space import and assembly protein 40 |
| NCBI Synonym Full Names: | coiled-coil-helix-coiled-coil-helix domain containing 4 |
| NCBI Official Symbol: | CHCHD4 |
| NCBI Official Synonym Symbols: | MIA40; TIMM40 |
| NCBI Protein Information: | mitochondrial intermembrane space import and assembly protein 40 |
| UniProt Protein Name: | Mitochondrial intermembrane space import and assembly protein 40 |
| UniProt Synonym Protein Names: | Coiled-coil-helix-coiled-coil-helix domain-containing protein 4 |
| Protein Family: | Mitochondrial intermembrane space import and assembly protein |
| UniProt Gene Name: | CHCHD4 |
| UniProt Entry Name: | MIA40_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CHCHD4 antibody (CAB13139) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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