Anti-CERK Antibody (CAB15889)
- SKU:
- CAB15889
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-CERK Antibody |
| 抗体コード: | CAB15889 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human CERK (NP_073603.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | LO2, A-549, Mouse testis, Rat thymus |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human CERK (NP_073603.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | CRRS PRGL SPAA HLGD GSSD LILI RKCS RFNF LRFL IRHT NQQD QFDF TFVE VYRV KKFQ FTSK HMED EDSD LKEG GKKR FGHI CSSH PSCC CTVS NSSW NCDG EVLH SPAI EVRV HCQL VRLF ARGI EENP KPDS HS |
| 遺伝子ID: | 64781 |
| Uniprot: | Q8TCT0 |
| セルラーロケーション: | Cytoplasm, Membrane, Peripheral membrane protein |
| 計算された分子量: | 37kDa/59kDa |
| 観察された分子量: | 60kDa |
| 同義語: | CERK, LK4, dA59H18.2, dA59H18.3, hCERK |
| バックグラウンド: | CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]). |
| UniProt Protein Function: | CERK: Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides. |
| UniProt Protein Details: | Protein type:EC 2.7.1.138; Kinase, lipid; Lipid Metabolism - sphingolipid Chromosomal Location of Human Ortholog: 22q13.31 Cellular Component: integral to membrane; plasma membrane Molecular Function:ceramide kinase activity; magnesium ion binding; protein binding Biological Process: ceramide metabolic process; glycosphingolipid metabolic process |
| NCBI Summary: | CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q8TCT0 |
| NCBI GenInfo Identifier: | 30172885 |
| NCBI Gene ID: | 64781 |
| NCBI Accession: | Q8TCT0.1 |
| UniProt Secondary Accession: | Q8TCT0,Q6NX59, Q9BYB3, Q9UGE5, A0JNT4, A8K611, |
| UniProt Related Accession: | Q8TCT0 |
| Molecular Weight: | 60kDa |
| NCBI Full Name: | Ceramide kinase |
| NCBI Synonym Full Names: | ceramide kinase |
| NCBI Official Symbol: | CERK |
| NCBI Official Synonym Symbols: | LK4; hCERK; dA59H18.2; dA59H18.3 |
| NCBI Protein Information: | ceramide kinase |
| UniProt Protein Name: | Ceramide kinase |
| UniProt Synonym Protein Names: | Acylsphingosine kinase; Lipid kinase 4; LK4 |
| Protein Family: | Ceramide kinase |
| UniProt Gene Name: | CERK |
 | Western blot analysis of extracts of various cell lines, using CERK antibody (CAB15889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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