Anti-CDK6 Antibody (RACO0273)
- SKU:
- RACO0273
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- IP
- Application:
- WB
- Application:
- ELISA
- Conjugation:
- Unconjugated
Description
| 商品名: | CDK6 Antibody |
| Product SKU: | RACO0273 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IP |
| 推奨される希釈: | WB:1:500-1:5000, IP:1:200-1:1000 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human CDK6 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases (PubMed:23918663). |
| シノニム: | Cyclin-dependent kinase 6 (EC 2.7.11.22) (Cell division protein kinase 6) (Serine/threonine-protein kinase PLSTIRE), CDK6, CDKN6 |
 | Immunoprecipitating CDK6 in K562 whole cell lysate Lane 1: Rabbit control IgG instead of RACO0273 in K562 whole cell lysate For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: RACO0273(2µg)+ K562 whole cell lysate (500µg). Lane 3: K562 whole cell lysate (10µg). |
 | Western Blot. Positive WB detected in Hela whole cell lysate Jurkat whole cell lysate K562 whole cell lysate 293 whole cell lysate All lanes CDK6 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 37 kDa. Observed band size: 37 kDa. |
| UniProt Protein Function: | CDK6: a protein kinase of the CDK family. Important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. Phosphorylates and regulates the activity of the Rb tumor suppressor protein. Overexpressed and/or disrupted by translocation in leukemias, lymphomas and other cancers and amplified in gliomas and rodent cancers. |
| UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.22; Protein kinase, CMGC; Cell cycle regulation; CMGC group; CDK family; CDK/CDK4 subfamily; CDK4 subfamily Chromosomal Location of Human Ortholog: 7q21-q22 Cellular Component: centrosome; cyclin-dependent protein kinase holoenzyme complex; cytoplasm; cytosol; nucleoplasm; nucleus; ruffle Molecular Function:ATP binding; cyclin binding; cyclin-dependent protein kinase activity; protein binding Biological Process: astrocyte development; cell cycle arrest; cell dedifferentiation; dentate gyrus development; G1/S transition of mitotic cell cycle; generation of neurons; gliogenesis; lateral ventricle development; negative regulation of cell cycle; negative regulation of cell differentiation; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of myeloid cell differentiation; negative regulation of osteoblast differentiation; positive regulation of cell-matrix adhesion; positive regulation of fibroblast proliferation; protein amino acid phosphorylation; regulation of erythrocyte differentiation; regulation of gene expression; response to virus Disease: Microcephaly 12, Primary, Autosomal Recessive |
| NCBI Summary: | The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009] |
| UniProt Code: | Q00534 |
| NCBI GenInfo Identifier: | 223718134 |
| NCBI Gene ID: | 1021 |
| NCBI Accession: | NP_001138778.1 |
| UniProt Secondary Accession: | Q00534,A4D1G0, |
| UniProt Related Accession: | Q00534 |
| Molecular Weight: | 36,938 Da |
| NCBI Full Name: | cyclin-dependent kinase 6 |
| NCBI Synonym Full Names: | cyclin dependent kinase 6 |
| NCBI Official Symbol: | CDK6 |
| NCBI Official Synonym Symbols: | MCPH12; PLSTIRE |
| NCBI Protein Information: | cyclin-dependent kinase 6 |
| UniProt Protein Name: | Cyclin-dependent kinase 6 |
| UniProt Synonym Protein Names: | Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE |
| Protein Family: | Cyclin-dependent kinase |
| UniProt Gene Name: | CDK6 |
| UniProt Entry Name: | CDK6_HUMAN |