Anti-CDK2AP1 Antibody (CAB4203)
- SKU:
- CAB4203
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-CDK2AP1 Antibody |
| 抗体コード: | CAB4203 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human CDK2AP1 (NP_004633.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | U-87MG, 22Rv1, 293T, HeLa |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human CDK2AP1 (NP_004633.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MSYK PNLA AHMP AAAL NAAG SVHS PSTS MATS SQYR QLLS DYGP PSLG YTQG TGNS QVPQ SKYA ELLA IIEE LGKE IRPT YAGS KSAM ERLK RGII HARG LVRE CLAE TERN ARS |
| 遺伝子ID: | 8099 |
| Uniprot: | O14519 |
| セルラーロケーション: | |
| 計算された分子量: | 9kDa/12kDa |
| 観察された分子量: | 12kDa |
| 同義語: | CDK2AP1, DOC1, DORC1, ST19, doc-1, p12DOC-1 |
| バックグラウンド: | The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. This gene thus plays a role in both cell-cycle and epigenetic regulation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | CDK2AP1: specific inhibitor of the cell-cycle kinase CDK2. Belongs to the CDK2AP family. |
| UniProt Protein Details: | Protein type:DNA replication; Tumor suppressor; Cell cycle regulation Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: perinuclear region of cytoplasm; nucleus Biological Process: positive regulation of protein amino acid phosphorylation; DNA-dependent DNA replication; cell cycle |
| NCBI Summary: | The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. This gene thus plays a role in both cell-cycle and epigenetic regulation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012] |
| UniProt Code: | O14519 |
| NCBI GenInfo Identifier: | 6014988 |
| NCBI Gene ID: | 8099 |
| NCBI Accession: | O14519.1 |
| UniProt Secondary Accession: | O14519,F5GYA4, |
| UniProt Related Accession: | O14519 |
| Molecular Weight: | 115 |
| NCBI Full Name: | Cyclin-dependent kinase 2-associated protein 1 |
| NCBI Synonym Full Names: | cyclin-dependent kinase 2 associated protein 1 |
| NCBI Official Symbol: | CDK2AP1 |
| NCBI Official Synonym Symbols: | DOC1; ST19; DORC1; doc-1; p12DOC-1 |
| NCBI Protein Information: | cyclin-dependent kinase 2-associated protein 1; Deleted in oral cancer-1; deleted in oral cancer 1; CDK2-associated protein 1; putative oral cancer suppressor |
| UniProt Protein Name: | Cyclin-dependent kinase 2-associated protein 1 |
| UniProt Synonym Protein Names: | Deleted in oral cancer 1; DOC-1; Putative oral cancer suppressor |
| Protein Family: | Cyclin-dependent kinase 2-associated protein |
| UniProt Gene Name: | CDK2AP1 |
| UniProt Entry Name: | CDKA1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CDK2AP1 antibody (CAB4203) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
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