Anti-CDCA8 Antibody (CAB0675)
- SKU:
- CAB0675
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-CDCA8 Antibody |
| 抗体コード: | CAB0675 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant protein of human CDCA8 |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HL-60, HeLa, MCF7, Mouse spleen |
| 免疫原: | Recombinant protein of human CDCA8 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 55143 |
| Uniprot: | Q53HL2 |
| セルラーロケーション: | Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, nucleolus, spindle |
| 計算された分子量: | 31kDa |
| 観察された分子量: | 36-38kDa |
| 同義語: | CDCA8, BOR, BOREALIN, DasraB, MESRGP, borealin |
| バックグラウンド: | This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16. |
| UniProt Protein Function: | Borealin: Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. Belongs to the borealin family. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Nucleolus Chromosomal Location of Human Ortholog: 1p34.3 Cellular Component: intercellular bridge; protein complex; nucleolus; spindle; midbody; nucleus; cytosol; chromosome, pericentric region; chromocenter Molecular Function:protein binding Biological Process: cell division; mitotic cell cycle; mitotic metaphase plate congression; chromosome organization and biogenesis |
| NCBI Summary: | This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16. [provided by RefSeq, Apr 2013] |
| UniProt Code: | Q53HL2 |
| NCBI GenInfo Identifier: | 110832774 |
| NCBI Gene ID: | 55143 |
| NCBI Accession: | Q53HL2.2 |
| UniProt Secondary Accession: | Q53HL2,Q53HN1, Q96AM3, Q9NVW5, D3DPT4, |
| UniProt Related Accession: | Q53HL2 |
| Molecular Weight: | 280 |
| NCBI Full Name: | Borealin |
| NCBI Synonym Full Names: | cell division cycle associated 8 |
| NCBI Official Symbol: | CDCA8 |
| NCBI Official Synonym Symbols: | BOR; DasraB; MESRGP; BOREALIN |
| NCBI Protein Information: | borealin; Dasra B; dasra-B; hDasra-B; cell division cycle-associated protein 8; pluripotent embryonic stem cell-related gene 3 protein |
| UniProt Protein Name: | Borealin |
| UniProt Synonym Protein Names: | Cell division cycle-associated protein 8; Dasra-B; hDasra-B; Pluripotent embryonic stem cell-related gene 3 protein |
| UniProt Gene Name: | CDCA8 |
| UniProt Entry Name: | BOREA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CDCA8 antibody (CAB0675) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using CDCA8 antibody (CAB0675) (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat kidney using CDCA8 antibody (CAB0675) (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophagus using CDCA8 antibody (CAB0675) (40x lens). |