Anti-CDC37 Antibody (RACO0285)
- SKU:
- RACO0285
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- FC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | CDC37 Antibody |
| Product SKU: | RACO0285 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, FC |
| 推奨される希釈: | WB:1:500-1:5000, FC:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human Cdc37 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity (PubMed:8666233). Inhibits HSP90AA1 ATPase activity (PubMed:23569206). |
| シノニム: | Hsp90 co-chaperone Cdc37 (Hsp90 chaperone protein kinase-targeting subunit) (p50Cdc37) [Cleaved into: Hsp90 co-chaperone Cdc37, N-terminally processed], CDC37, CDC37A |
 | Overlay histogram showing Hela cells stained with RACO0285 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg) 1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg) 1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
 | Western Blot. Positive WB detected in Hela whole cell lysate Jurkat whole cell lysate MCF-7 whole cell lysate 293 whole cell lysate K562 whole cell lysate PC-3 whole cell lysate All lanes CDC37 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 45 kDa. Observed band size: 50 kDa. |
| UniProt Protein Function: | CDC37: a molecular chaperone that functions in cell signal transduction. Forms complexes with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. Also interacts with androgen receptor. It is thought to play a critical role in directing Hsp90 to its target kinases. |
| UniProt Protein Details: | Protein type:Chaperone Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: protein complex; cytoplasm; cytosol Molecular Function:protein kinase B binding; protein binding; chaperone binding; heat shock protein binding; unfolded protein binding; mitogen-activated protein kinase kinase kinase binding; Hsp90 protein binding; kinase binding Biological Process: protein folding; protein stabilization; regulation of cyclin-dependent protein kinase activity; protein targeting |
| NCBI Summary: | The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q16543 |
| NCBI GenInfo Identifier: | 21542000 |
| NCBI Gene ID: | 11140 |
| NCBI Accession: | Q16543.1 |
| UniProt Secondary Accession: | Q16543,Q53YA2, |
| UniProt Related Accession: | Q16543 |
| Molecular Weight: | 378 |
| NCBI Full Name: | Hsp90 co-chaperone Cdc37 |
| NCBI Synonym Full Names: | cell division cycle 37 |
| NCBI Official Symbol: | CDC37 |
| NCBI Official Synonym Symbols: | P50CDC37 |
| NCBI Protein Information: | hsp90 co-chaperone Cdc37; cell division cycle 37 homolog; CDC37 cell division cycle 37 homolog; hsp90 chaperone protein kinase-targeting subunit; CDC37 (cell division cycle 37, S. cerevisiae, homolog) |
| UniProt Protein Name: | Hsp90 co-chaperone Cdc37 |
| UniProt Synonym Protein Names: | Hsp90 chaperone protein kinase-targeting subunit; p50Cdc37Hsp90 co-chaperone Cdc37, N-terminally processed |
| Protein Family: | Hsp90 co-chaperone |
| UniProt Gene Name: | CDC37 |
| UniProt Entry Name: | CDC37_HUMAN |