Anti-CDC23 Antibody (CAB15116)
- SKU:
- CAB15116
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-CDC23 Antibody |
| 抗体コード: | CAB15116 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CDC23 (NP_004652.2). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-87MG, 293T, HeLa, Jurkat, HepG2, NIH/3T3, mouse liver, mouse kidney, rat liver |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CDC23 (NP_004652.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | HSSK WSAE LAFS LPAL PLAE LQPP PPIT EEDA QDMD AYTL AKAY FDVK EYDR AAHF LHGC NSKK AYFL YMYS RYLS GEKK KDDE TVDS LGPL EKGQ VKNE A |
| 遺伝子ID: | 8697 |
| Uniprot: | Q9UJX2 |
| セルラーロケーション: | |
| 計算された分子量: | 16kDa/55kDa/68kDa |
| 観察された分子量: | 69kDa |
| 同義語: | CDC23, ANAPC8, APC8, CUT23 |
| バックグラウンド: | The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. |
| UniProt Protein Function: | CDC23: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC8/CDC23 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 5q31 Cellular Component: nucleoplasm; anaphase-promoting complex; intracellular; cytosol Molecular Function:ubiquitin-protein ligase activity Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; regulation of mitotic metaphase/anaphase transition; mitotic cell cycle spindle assembly checkpoint; regulation of exit from mitosis; mitotic cell cycle; mitotic metaphase plate congression; mitotic metaphase/anaphase transition |
| NCBI Summary: | The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UJX2 |
| NCBI GenInfo Identifier: | 254763423 |
| NCBI Gene ID: | 8697 |
| NCBI Accession: | Q9UJX2.3 |
| UniProt Secondary Accession: | Q9UJX2,O75433, Q53FN2, Q9BS73, A8K6E5, B4E3A2, B7WP05 D3DQB7, |
| UniProt Related Accession: | Q9UJX2 |
| Molecular Weight: | 55,882 Da |
| NCBI Full Name: | Cell division cycle protein 23 homolog |
| NCBI Synonym Full Names: | cell division cycle 23 |
| NCBI Official Symbol: | CDC23 |
| NCBI Official Synonym Symbols: | APC8; CUT23; ANAPC8 |
| NCBI Protein Information: | cell division cycle protein 23 homolog; cyclosome subunit 8; cell division cycle 23 homolog; anaphase promoting complex subunit 8; anaphase-promoting complex subunit 8 |
| UniProt Protein Name: | Cell division cycle protein 23 homolog |
| UniProt Synonym Protein Names: | Anaphase-promoting complex subunit 8; APC8; Cyclosome subunit 8 |
| Protein Family: | Anaphase-promoting complex |
| UniProt Gene Name: | CDC23 |
| UniProt Entry Name: | CDC23_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CDC23 antibody (CAB15116) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat spleen using CDC23 antibody (CAB15116) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human tonsil using CDC23 antibody (CAB15116) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using CDC23 antibody (CAB15116) at dilution of 1:100 (40x lens). |
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